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2pnh

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[[Image:2pnh.jpg|left|200px]]
[[Image:2pnh.jpg|left|200px]]
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{{Structure
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|GENE= priB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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{{STRUCTURE_2pnh| PDB=2pnh | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pnh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pnh OCA], [http://www.ebi.ac.uk/pdbsum/2pnh PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2pnh RCSB]</span>
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'''Escherichia coli PriB E39A variant'''
'''Escherichia coli PriB E39A variant'''
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[[Category: Keck, J L.]]
[[Category: Keck, J L.]]
[[Category: Lopper, M E.]]
[[Category: Lopper, M E.]]
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[[Category: beta barrel]]
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[[Category: Beta barrel]]
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[[Category: dna binding protein]]
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[[Category: Dna binding protein]]
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[[Category: ob fold]]
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[[Category: Ob fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 13:28:49 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:38:18 2008''
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Revision as of 10:28, 4 May 2008

Image:2pnh.jpg

Template:STRUCTURE 2pnh

Escherichia coli PriB E39A variant


Overview

Collapsed DNA replication forks must be reactivated through origin-independent reloading of the replication machinery (replisome) to ensure complete duplication of cellular genomes. In E. coli, the PriA-dependent pathway is the major replication restart mechanism and requires primosome proteins PriA, PriB, and DnaT for replisome reloading. However, the molecular mechanisms that regulate origin-independent replisome loading are not fully understood. Here, we demonstrate that assembly of primosome protein complexes represents a key regulatory mechanism, as inherently weak PriA-PriB and PriB-DnaT interactions are strongly stimulated by single-stranded DNA. Furthermore, the binding site on PriB for single-stranded DNA partially overlaps the binding sites for PriA and DnaT, suggesting a dynamic primosome assembly process in which single-stranded DNA is handed off from one primosome protein to another as a repaired replication fork is reactivated. This model helps explain how origin-independent initiation of DNA replication is restricted to repaired replication forks, preventing overreplication of the genome.

About this Structure

2PNH is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

A hand-off mechanism for primosome assembly in replication restart., Lopper M, Boonsombat R, Sandler SJ, Keck JL, Mol Cell. 2007 Jun 22;26(6):781-93. PMID:17588514 Page seeded by OCA on Sun May 4 13:28:49 2008

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