2n77

From Proteopedia

(Difference between revisions)

Revision as of 15:41, 8 June 2021

NMR solution structure of a complex of PEP-19 bound to the C-domain of apo calmodulin

Structural highlights

2n77 is a 2 chain structure with sequence from Human. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	CALM1, CALM, CAM, CAM1, CALM2, CAM2, CAMB, CALM3, CALML2, CAM3, CAMC, CAMIII (HUMAN), PCP4, PEP19 (HUMAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[PCP4_HUMAN] Probable regulator of calmodulin signaling.^[1]

Publication Abstract from PubMed

PEP-19 is a small protein that increases the rates of Ca2+ binding to the C-domain of calmodulin (CaM) by an unknown mechanism. Although an IQ motif promotes binding to CaM, an acidic sequence in PEP-19 is required to modulate Ca2+ binding and to sensitize HeLa cells to ATP-induced Ca2+ release. Here, we report the NMR solution structure of a complex between PEP-19 and the C-domain of apo CaM. The acidic sequence of PEP-19 associates between helices E and F of CaM via hydrophobic interactions. This allows the acidic side chains in PEP-19 to extend toward the solvent and form a negatively charged surface that resembles a catcher's mitt near Ca2+ binding loop III of CaM. The topology and gradients of negative electrostatic surface potential support a mechanism by which PEP-19 increases the rate of Ca2+ binding to the C-domain of CaM by 'catching' and electrostatically steering Ca2+ to site III.

PEP-19 modulates calcium binding to calmodulin by electrostatic steering.,Wang X, Putkey JA Nat Commun. 2016 Nov 23;7:13583. doi: 10.1038/ncomms13583. PMID:27876793^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kleerekoper QK, Putkey JA. PEP-19, an intrinsically disordered regulator of calmodulin signaling. J Biol Chem. 2009 Mar 20;284(12):7455-64. doi: 10.1074/jbc.M808067200. Epub 2008 , Dec 23. PMID:19106096 doi:http://dx.doi.org/10.1074/jbc.M808067200
↑ Wang X, Putkey JA. PEP-19 modulates calcium binding to calmodulin by electrostatic steering. Nat Commun. 2016 Nov 23;7:13583. doi: 10.1038/ncomms13583. PMID:27876793 doi:http://dx.doi.org/10.1038/ncomms13583

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2n77"

@@ Line 1: / Line 1: @@
 ==NMR solution structure of a complex of PEP-19 bound to the C-domain of apo calmodulin==
-<StructureSection load='2n77' size='340' side='right' caption='[[2n77]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='2n77' size='340' side='right'caption='[[2n77]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2n77]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N77 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2N77 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2n77]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N77 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N77 FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CALM1, CALM, CAM, CAM1, CALM2, CAM2, CAMB, CALM3, CALML2, CAM3, CAMC, CAMIII ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), PCP4, PEP19 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CALM1, CALM, CAM, CAM1, CALM2, CAM2, CAMB, CALM3, CALML2, CAM3, CAMC, CAMIII ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), PCP4, PEP19 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2n77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n77 OCA], [http://pdbe.org/2n77 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2n77 RCSB], [http://www.ebi.ac.uk/pdbsum/2n77 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2n77 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n77 OCA], [https://pdbe.org/2n77 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n77 RCSB], [https://www.ebi.ac.uk/pdbsum/2n77 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n77 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/PCP4_HUMAN PCP4_HUMAN]] Probable regulator of calmodulin signaling.<ref>PMID:19106096</ref>
+[[https://www.uniprot.org/uniprot/PCP4_HUMAN PCP4_HUMAN]] Probable regulator of calmodulin signaling.<ref>PMID:19106096</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 23: @@
 </StructureSection>
 [[Category: Human]]
+[[Category: Large Structures]]
 [[Category: Putkey, J A]]
 [[Category: Wang, X]]

2n77

From Proteopedia

Revision as of 15:41, 8 June 2021

NMR solution structure of a complex of PEP-19 bound to the C-domain of apo calmodulin

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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