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2v3c

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Revision as of 11:18, 17 June 2020

Crystal structure of the SRP54-SRP19-7S.S SRP RNA complex of M. jannaschii

Structural highlights

2v3c is a 6 chain structure with sequence from Atcc 43067. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1l9a, 1lng
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SRP19_METJA] Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds directly to 7S RNA and mediates binding of the 54 kDa subunit of the SRP (By similarity).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The signal-recognition particle (SRP) is a ubiquitous protein-RNA complex that targets proteins to cellular membranes for insertion or secretion. A key player in SRP-mediated protein targeting is the evolutionarily conserved core consisting of the SRP RNA and the multidomain protein SRP54. Communication between the SRP54 domains is critical for SRP function, where signal sequence binding at the M domain directs receptor binding at the GTPase domain (NG domain). These SRP activities are linked to domain rearrangements, for which the role of SRP RNA is not clear. In free SRP, a direct interaction of the GTPase domain with SRP RNA has been proposed but has never been structurally verified. In this study, we present the crystal structure at 2.5-A resolution of the SRP54-SRP19-SRP RNA complex of Methanococcus jannaschii SRP. The structure reveals an RNA-bound conformation of the SRP54 GTPase domain, in which the domain is spatially well separated from the signal peptide binding site. The association of both the N and G domains with SRP RNA in free SRP provides further structural evidence for the pivotal role of SRP RNA in the regulation of the SRP54 activity.

Interaction of signal-recognition particle 54 GTPase domain and signal-recognition particle RNA in the free signal-recognition particle.,Hainzl T, Huang S, Sauer-Eriksson AE Proc Natl Acad Sci U S A. 2007 Sep 18;104(38):14911-6. Epub 2007 Sep 10. PMID:17846429^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hainzl T, Huang S, Sauer-Eriksson AE. Interaction of signal-recognition particle 54 GTPase domain and signal-recognition particle RNA in the free signal-recognition particle. Proc Natl Acad Sci U S A. 2007 Sep 18;104(38):14911-6. Epub 2007 Sep 10. PMID:17846429 doi:0702467104

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2v3c"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the SRP54-SRP19-7S.S SRP RNA complex of M. jannaschii==
-<StructureSection load='2v3c' size='340' side='right' caption='[[2v3c]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
+<StructureSection load='2v3c' size='340' side='right'caption='[[2v3c]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2v3c]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43067 Atcc 43067]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V3C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2V3C FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2v3c]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43067 Atcc 43067]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V3C OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2V3C FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1l9a|1l9a]], [[1lng|1lng]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v3c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v3c OCA], [http://pdbe.org/2v3c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2v3c RCSB], [http://www.ebi.ac.uk/pdbsum/2v3c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2v3c ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2v3c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v3c OCA], [http://pdbe.org/2v3c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2v3c RCSB], [http://www.ebi.ac.uk/pdbsum/2v3c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2v3c ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 36: / Line 36: @@
 </StructureSection>
 [[Category: Atcc 43067]]
+[[Category: Large Structures]]
 [[Category: Hainzl, T]]
 [[Category: Huang, S]]

2v3c

From Proteopedia

Revision as of 11:18, 17 June 2020

Crystal structure of the SRP54-SRP19-7S.S SRP RNA complex of M. jannaschii

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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