2pz8

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[[Image:2pz8.jpg|left|200px]]
[[Image:2pz8.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2pz8 |SIZE=350|CAPTION= <scene name='initialview01'>2pz8</scene>, resolution 2.00&Aring;
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The line below this paragraph, containing "STRUCTURE_2pz8", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=APC:DIPHOSPHOMETHYLPHOSPHONIC+ACID+ADENOSYL+ESTER'>APC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/NAD(+)_synthase NAD(+) synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.1.5 6.3.1.5] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= nadE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 Bacillus anthracis])
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-->
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|DOMAIN=
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{{STRUCTURE_2pz8| PDB=2pz8 | SCENE= }}
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|RELATEDENTRY=[[2pza|2PZA]], [[2pzb|2PZB]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pz8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pz8 OCA], [http://www.ebi.ac.uk/pdbsum/2pz8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2pz8 RCSB]</span>
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}}
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'''NAD+ Synthetase from Bacillus anthracis with AMP-CPP and Mg2+'''
'''NAD+ Synthetase from Bacillus anthracis with AMP-CPP and Mg2+'''
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Structural adaptation of an interacting non-native C-terminal helical extension revealed in the crystal structure of NAD+ synthetase from Bacillus anthracis., McDonald HM, Pruett PS, Deivanayagam C, Protasevich II, Carson WM, DeLucas LJ, Brouillette WJ, Brouillette CG, Acta Crystallogr D Biol Crystallogr. 2007 Aug;63(Pt 8):891-905. Epub 2007, Jul 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17642516 17642516]
Structural adaptation of an interacting non-native C-terminal helical extension revealed in the crystal structure of NAD+ synthetase from Bacillus anthracis., McDonald HM, Pruett PS, Deivanayagam C, Protasevich II, Carson WM, DeLucas LJ, Brouillette WJ, Brouillette CG, Acta Crystallogr D Biol Crystallogr. 2007 Aug;63(Pt 8):891-905. Epub 2007, Jul 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17642516 17642516]
[[Category: Bacillus anthracis]]
[[Category: Bacillus anthracis]]
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[[Category: NAD(+) synthase]]
 
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Brouillette, C G.]]
[[Category: Brouillette, C G.]]
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[[Category: Protasevich, I I.]]
[[Category: Protasevich, I I.]]
[[Category: Pruett, P S.]]
[[Category: Pruett, P S.]]
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[[Category: his-tag]]
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[[Category: His-tag]]
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[[Category: ligase]]
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[[Category: Ligase]]
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[[Category: protein-substrate analog complex]]
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[[Category: Protein-substrate analog complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 14:03:04 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:42:35 2008''
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Revision as of 11:03, 4 May 2008

Image:2pz8.jpg

Template:STRUCTURE 2pz8

NAD+ Synthetase from Bacillus anthracis with AMP-CPP and Mg2+


Overview

The crystal structures of NH(3)-dependent NAD+ synthetase from Bacillus anthracis as the apoenzyme (1.9 A), in complex with the natural catalytic products AMP and pyrophosphate (2.4 A) and in complex with the substrate analog adenosine 5'-(alpha,beta-methylene)triphosphate (2.0 A) have been determined. NAD+ synthetase catalyzes the last step in the biosynthesis of the vitally important cofactor NAD+. In comparison to other NAD+ synthetase crystal structures, the C-terminal His-tagged end of the apoenzyme adopts a novel helical conformation, causing significant compensatory changes in the region. The structural accommodations observed in B. anthracis NAD+ synthetase are remarkable in the absence of adverse affects on enzyme activity. They also illustrate a rare example of the influence of a non-native C-terminal His-tag extension on the structure of a native protein. In contrast to the apoenzyme, when AMP and pyrophosphate or adenosine 5'-(alpha,beta-methylene)triphosphate are bound, the C-terminus adopts a conformation that allows ATP binding and overall the structure then resembles other NAD+ synthetase structures. The structures of NAD+ synthetase complexes from B. anthracis are compared with published X-ray crystal structures of the enzyme from B. subtilis, Escherichia coli and Helicobacter pylori. These comparisons support the novel observation that P1 and P2 loop ordering is not a consequence of crystal contacts but rather a consequence of intrinsic intramolecular interactions within the ordered subunit.

About this Structure

2PZ8 is a Single protein structure of sequence from Bacillus anthracis. Full crystallographic information is available from OCA.

Reference

Structural adaptation of an interacting non-native C-terminal helical extension revealed in the crystal structure of NAD+ synthetase from Bacillus anthracis., McDonald HM, Pruett PS, Deivanayagam C, Protasevich II, Carson WM, DeLucas LJ, Brouillette WJ, Brouillette CG, Acta Crystallogr D Biol Crystallogr. 2007 Aug;63(Pt 8):891-905. Epub 2007, Jul 17. PMID:17642516 Page seeded by OCA on Sun May 4 14:03:04 2008

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