2uy5

From Proteopedia

(Difference between revisions)

Revision as of 11:13, 17 June 2020

ScCTS1_kinetin crystal structure

Structural highlights

2uy5 is a 1 chain structure with sequence from Atcc 18824. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	2uy2, 2uy3, 2uy4
Activity:	Chitinase, with EC number 3.2.1.14
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CHIT_YEAST] Chitinase is required for cell separation during growth of Saccharomyces cerevisiae.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Chitinases hydrolyse the beta(1,4)-glycosidic bonds of chitin, an essential fungal cell wall component. Genetic data on a subclass of fungal family 18 chitinases have suggested a role in cell wall morphology. Specific inhibitors of these enzymes would be useful as tools to study their role in cell wall morphogenesis and could possess antifungal properties. Here, we describe the crystallographic structure of a fungal "plant-type" family 18 chitinase, that of Saccharomyces cerevisiae CTS1. The enzyme is active against 4-methylumbelliferyl chitooligosaccharides and displays an unusually low pH optimum for activity. A library screen against ScCTS1 yielded hits with Ki 's as low as 3.2 microM. Crystal structures of ScCTS1 in complex with inhibitors from three series reveal striking mimicry of carbohydrate substrate by small aromatic moieties and a pocket that could be further exploited in optimization of these inhibitors.

Structure of Saccharomyces cerevisiae chitinase 1 and screening-based discovery of potent inhibitors.,Hurtado-Guerrero R, van Aalten DM Chem Biol. 2007 May;14(5):589-99. PMID:17524989^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hurtado-Guerrero R, van Aalten DM. Structure of Saccharomyces cerevisiae chitinase 1 and screening-based discovery of potent inhibitors. Chem Biol. 2007 May;14(5):589-99. PMID:17524989 doi:S1074-5521(07)00112-3

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2uy5"

@@ Line 1: / Line 1: @@
 ==ScCTS1_kinetin crystal structure==
-<StructureSection load='2uy5' size='340' side='right' caption='[[2uy5]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
+<StructureSection load='2uy5' size='340' side='right'caption='[[2uy5]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2uy5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UY5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2UY5 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2uy5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UY5 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2UY5 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=H35:N-(FURAN-2-YLMETHYL)-7H-PURIN-6-AMINE'>H35</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=H35:N-(FURAN-2-YLMETHYL)-7H-PURIN-6-AMINE'>H35</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2uy2|2uy2]], [[2uy3|2uy3]], [[2uy4|2uy4]]</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2uy5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uy5 OCA], [http://pdbe.org/2uy5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2uy5 RCSB], [http://www.ebi.ac.uk/pdbsum/2uy5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2uy5 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2uy5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uy5 OCA], [http://pdbe.org/2uy5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2uy5 RCSB], [http://www.ebi.ac.uk/pdbsum/2uy5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2uy5 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 32: / Line 32: @@
 ==See Also==
-*[[Chitinase|Chitinase]]
+*[[Chitinase 3D structures|Chitinase 3D structures]]
 == References ==
 <references/>
@@ Line 39: / Line 39: @@
 [[Category: Atcc 18824]]
 [[Category: Chitinase]]
+[[Category: Large Structures]]
 [[Category: Aalten, D M.F van]]
 [[Category: Hurtado-Guerrero, R]]

2uy5

From Proteopedia

Revision as of 11:13, 17 June 2020

ScCTS1_kinetin crystal structure

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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