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2q2u
From Proteopedia
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[[Image:2q2u.gif|left|200px]] | [[Image:2q2u.gif|left|200px]] | ||
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'''Structure of Chlorella virus DNA ligase-product DNA complex''' | '''Structure of Chlorella virus DNA ligase-product DNA complex''' | ||
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[[Category: Shuman, S.]] | [[Category: Shuman, S.]] | ||
[[Category: Smith, P.]] | [[Category: Smith, P.]] | ||
| - | [[Category: | + | [[Category: Ligase]] |
| - | [[Category: | + | [[Category: Ligase/dna complex]] |
| - | [[Category: | + | [[Category: Product-complex]] |
| - | [[Category: | + | [[Category: Protein-dna complex]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 14:13:13 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 11:13, 4 May 2008
Structure of Chlorella virus DNA ligase-product DNA complex
Overview
Chlorella virus DNA ligase, the smallest eukaryotic ligase known, has pluripotent biological activity and an intrinsic nick-sensing function, despite having none of the accessory domains found in cellular ligases. A 2.3-A crystal structure of the Chlorella virus ligase-AMP intermediate bound to duplex DNA containing a 3'-OH-5'-PO4 nick reveals a new mode of DNA envelopment, in which a short surface loop emanating from the OB domain forms a beta-hairpin 'latch' that inserts into the DNA major groove flanking the nick. A network of interactions with the 3'-OH and 5'-PO4 termini in the active site illuminates the DNA adenylylation mechanism and the crucial roles of AMP in nick sensing and catalysis. Addition of a divalent cation triggered nick sealing in crystallo, establishing that the nick complex is a bona fide intermediate in the DNA repair pathway.
About this Structure
2Q2U is a Single protein structure of sequence from Paramecium bursaria chlorella virus 1. Full crystallographic information is available from OCA.
Reference
Structural basis for nick recognition by a minimal pluripotent DNA ligase., Nair PA, Nandakumar J, Smith P, Odell M, Lima CD, Shuman S, Nat Struct Mol Biol. 2007 Aug;14(8):770-8. Epub 2007 Jul 8. PMID:17618295 Page seeded by OCA on Sun May 4 14:13:13 2008
