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2q5i

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[[Image:2q5i.jpg|left|200px]]
[[Image:2q5i.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2q5i |SIZE=350|CAPTION= <scene name='initialview01'>2q5i</scene>, resolution 2.80&Aring;
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The line below this paragraph, containing "STRUCTURE_2q5i", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glycine--tRNA_ligase Glycine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.14 6.1.1.14] </span>
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|GENE= GARS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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|DOMAIN=
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{{STRUCTURE_2q5i| PDB=2q5i | SCENE= }}
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|RELATEDENTRY=[[2q5h|2Q5H]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2q5i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q5i OCA], [http://www.ebi.ac.uk/pdbsum/2q5i PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2q5i RCSB]</span>
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'''Crystal structure of apo S581L Glycyl-tRNA synthetase mutant'''
'''Crystal structure of apo S581L Glycyl-tRNA synthetase mutant'''
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[[Category: Stammers, D K.]]
[[Category: Stammers, D K.]]
[[Category: Talbot, K.]]
[[Category: Talbot, K.]]
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[[Category: aminoacyl-trna synthetase]]
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[[Category: Aminoacyl-trna synthetase]]
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[[Category: atp-binding]]
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[[Category: Atp-binding]]
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[[Category: glycyl-trna synthetase]]
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[[Category: Glycyl-trna synthetase]]
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[[Category: oppf]]
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[[Category: Oppf]]
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[[Category: oxford protein production facility]]
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[[Category: Oxford protein production facility]]
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[[Category: structural genomic]]
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[[Category: Structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 14:23:41 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:45:04 2008''
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Revision as of 11:23, 4 May 2008

Image:2q5i.jpg

Template:STRUCTURE 2q5i

Crystal structure of apo S581L Glycyl-tRNA synthetase mutant


Contents

Overview

Dominant mutations in the ubiquitous enzyme glycyl-tRNA synthetase (GlyRS), including S581L, lead to motor nerve degeneration. We have determined crystal structures of wildtype and S581L-mutant human GlyRS. The S581L mutation is approximately 50A from the active site, and yet gives reduced aminoacylation activity. The overall structures of wildtype and S581L-GlyRS, including the active site, are very similar. However, residues 567-575 of the anticodon-binding domain shift position and in turn could indirectly affect glycine binding via the tRNA or alternatively inhibit conformational changes. Reduced enzyme activity may underlie neuronal degeneration, although a dominant-negative effect is more likely in this autosomal dominant disorder.

Disease

Known disease associated with this structure: Charcot-Marie-Tooth disease, type 2D OMIM:[600287], Neuropathy, distal hereditary motor, type V OMIM:[600287]

About this Structure

2Q5I is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of human wildtype and S581L-mutant glycyl-tRNA synthetase, an enzyme underlying distal spinal muscular atrophy., Cader MZ, Ren J, James PA, Bird LE, Talbot K, Stammers DK, FEBS Lett. 2007 Jun 26;581(16):2959-64. Epub 2007 May 29. PMID:17544401 Page seeded by OCA on Sun May 4 14:23:41 2008

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