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2vop

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Revision as of 08:16, 25 June 2020

Crystal structure of N-terminal domains of Human La protein complexed with RNA oligomer AUUUU

Structural highlights

2vop is a 2 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	1s7a, 1zh5, 1yty, 1s79, 2voo, 2vod, 2von, 1owx
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The eukaryotic La protein recognizes the 3' poly(U) sequences of nascent RNA polymerase III transcripts to assist folding and maturation. The 3' ends of such RNAs are bound by the N-terminal domain of La (LaNTD). We have solved the crystal structures of four LaNTD:RNA complexes, each containing a different single-stranded RNA oligomer, and compared them to the structure of a previously published LaNTD:RNA complex containing partially duplex RNA. The presence of purely single-stranded RNA in the binding pocket at the interface between the La motif and RRM domains allows significantly closer contact with the 3' end of the RNA. Comparison of the different LaNTD:RNA complexes identifies a conserved set of interactions with the last two nucleotides at the 3' end of the RNA ligand that are key to binding. Strikingly, we also observe two alternative conformations of bound ssRNA, indicative of an unexpected degree of plasticity in the modes of RNA binding.

Structural analysis reveals conformational plasticity in the recognition of RNA 3' ends by the human La protein.,Kotik-Kogan O, Valentine ER, Sanfelice D, Conte MR, Curry S Structure. 2008 Jun;16(6):852-62. PMID:18547518^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kotik-Kogan O, Valentine ER, Sanfelice D, Conte MR, Curry S. Structural analysis reveals conformational plasticity in the recognition of RNA 3' ends by the human La protein. Structure. 2008 Jun;16(6):852-62. PMID:18547518 doi:http://dx.doi.org/10.1016/j.str.2008.02.021

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2vop"

@@ Line 1: / Line 1: @@
 ==Crystal structure of N-terminal domains of Human La protein complexed with RNA oligomer AUUUU==
-<StructureSection load='2vop' size='340' side='right' caption='[[2vop]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
+<StructureSection load='2vop' size='340' side='right'caption='[[2vop]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2vop]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VOP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VOP FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2vop]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VOP OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2VOP FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1s7a|1s7a]], [[1zh5|1zh5]], [[1yty|1yty]], [[1s79|1s79]], [[2voo|2voo]], [[2vod|2vod]], [[2von|2von]], [[1owx|1owx]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vop FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vop OCA], [http://pdbe.org/2vop PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vop RCSB], [http://www.ebi.ac.uk/pdbsum/2vop PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2vop ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2vop FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vop OCA], [http://pdbe.org/2vop PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vop RCSB], [http://www.ebi.ac.uk/pdbsum/2vop PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2vop ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 32: / Line 32: @@
 </StructureSection>
 [[Category: Human]]
+[[Category: Large Structures]]
 [[Category: Conte, M R]]
 [[Category: Curry, S]]

2vop

From Proteopedia

Revision as of 08:16, 25 June 2020

Crystal structure of N-terminal domains of Human La protein complexed with RNA oligomer AUUUU

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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