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2vfy

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Revision as of 07:52, 25 June 2020

AKAP18 delta central domain

Structural highlights

2vfy is a 1 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	2vfk, 2vfl
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Protein kinase A anchoring proteins (AKAPs), defined by their capacity to target the cAMP-dependent protein kinase to distinct subcellular locations, function as molecular scaffolds mediating the assembly of multicomponent complexes to integrate and organise multiple signalling events. Despite their central importance in regulating cellular processes, little is known regarding their diverse structures and molecular mechanisms. Here, using bioinformatics and X-ray crystallography, we define a central domain of AKAP18 delta (AKAP18(CD)) as a member of the 2H phosphoesterase family. The domain features two conserved His-x-Thr motifs positioned at the base of a groove located between two lobes related by pseudo 2-fold symmetry. Nucleotide co-crystallisation screening revealed that this groove binds specifically to adenosine 5'-monophosphate (5'AMP) and cytosine 5'-monophosphate (5'CMP), with the affinity constant for AMP in the physiological concentration range. This is the first example of an AKAP capable of binding a small molecule. Our data generate two functional hypotheses for the AKAP18 central domain. It may act as a phosphoesterase, although we did not identify a substrate, or as an AMP sensor with the potential to couple intracellular AMP levels to PKA signalling events.

AKAP18 contains a phosphoesterase domain that binds AMP.,Gold MG, Smith FD, Scott JD, Barford D J Mol Biol. 2008 Feb 1;375(5):1329-43. Epub 2007 Nov 22. PMID:18082768^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Gold MG, Smith FD, Scott JD, Barford D. AKAP18 contains a phosphoesterase domain that binds AMP. J Mol Biol. 2008 Feb 1;375(5):1329-43. Epub 2007 Nov 22. PMID:18082768 doi:10.1016/j.jmb.2007.11.037

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2vfy"

@@ Line 1: / Line 1: @@
 ==AKAP18 delta central domain==
-<StructureSection load='2vfy' size='340' side='right' caption='[[2vfy]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
+<StructureSection load='2vfy' size='340' side='right'caption='[[2vfy]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2vfy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VFY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VFY FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2vfy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VFY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2VFY FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2vfk|2vfk]], [[2vfl|2vfl]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vfy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vfy OCA], [http://pdbe.org/2vfy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vfy RCSB], [http://www.ebi.ac.uk/pdbsum/2vfy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2vfy ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2vfy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vfy OCA], [http://pdbe.org/2vfy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vfy RCSB], [http://www.ebi.ac.uk/pdbsum/2vfy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2vfy ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 31: / Line 31: @@
 </StructureSection>
 [[Category: Human]]
+[[Category: Large Structures]]
 [[Category: Barford, D]]
 [[Category: Gold, M G]]

2vfy

From Proteopedia

Revision as of 07:52, 25 June 2020

AKAP18 delta central domain

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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