2w11

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(Difference between revisions)

Revision as of 08:46, 25 June 2020

Structure of the L-2-haloacid dehalogenase from Sulfolobus tokodaii

Structural highlights

2w11 is a 2 chain structure with sequence from Dsm 16993. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	2w43
Activity:	Hydrolase, with EC number 3.8.1.2
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

Haloacid dehalogenases have potential applications in the pharmaceutical and fine chemical industry as well as in the remediation of contaminated land. The L: -2-haloacid dehalogenase from the thermophilic archaeon Sulfolobus tokodaii has been cloned and over-expressed in Escherichia coli and successfully purified to homogeneity. Here we report the structure of the recombinant dehalogenase solved by molecular replacement in two different crystal forms. The enzyme is a homodimer with each monomer being composed of a core-domain of a beta-sheet bundle surrounded by alpha-helices and an alpha-helical sub-domain. This fold is similar to previously solved mesophilic L: -haloacid dehalogenase structures. The monoclinic crystal form contains a putative inhibitor L: -lactate in the active site. The enzyme displays haloacid dehalogenase activity towards carboxylic acids with the halide attached at the C2 position with the highest activity towards chloropropionic acid. The enzyme is thermostable with maximum activity at 60 degrees C and a half-life of over 1 h at 70 degrees C. The enzyme is relatively stable to solvents with 25% activity lost when incubated for 1 h in 20% v/v DMSO.

Biochemical and structural studies of a L: -haloacid dehalogenase from the thermophilic archaeon Sulfolobus tokodaii.,Rye CA, Isupov MN, Lebedev AA, Littlechild JA Extremophiles. 2008 Nov 29. PMID:19039518^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Rye CA, Isupov MN, Lebedev AA, Littlechild JA. Biochemical and structural studies of a L: -haloacid dehalogenase from the thermophilic archaeon Sulfolobus tokodaii. Extremophiles. 2008 Nov 29. PMID:19039518 doi:10.1007/s00792-008-0208-0

1 Structural highlights
2 Publication Abstract from PubMed
3 See Also
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2w11"

@@ Line 1: / Line 1: @@
 ==Structure of the L-2-haloacid dehalogenase from Sulfolobus tokodaii==
-<StructureSection load='2w11' size='340' side='right' caption='[[2w11]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
+<StructureSection load='2w11' size='340' side='right'caption='[[2w11]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2w11]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Dsm_16993 Dsm 16993]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W11 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2W11 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2w11]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Dsm_16993 Dsm 16993]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W11 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2W11 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2OP:(2S)-2-HYDROXYPROPANOIC+ACID'>2OP</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2OP:(2S)-2-HYDROXYPROPANOIC+ACID'>2OP</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2w43|2w43]]</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hydrolase Hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.8.1.2 3.8.1.2] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w11 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w11 OCA], [http://pdbe.org/2w11 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2w11 RCSB], [http://www.ebi.ac.uk/pdbsum/2w11 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2w11 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2w11 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w11 OCA], [http://pdbe.org/2w11 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2w11 RCSB], [http://www.ebi.ac.uk/pdbsum/2w11 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2w11 ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">
@@ Line 20: / Line 20: @@
 ==See Also==
-*[[Dehalogenase|Dehalogenase]]
+*[[Dehalogenase 3D structures|Dehalogenase 3D structures]]
 == References ==
 <references/>
@@ Line 27: / Line 27: @@
 [[Category: Dsm 16993]]
 [[Category: Hydrolase]]
+[[Category: Large Structures]]
 [[Category: Isupov, M N]]
 [[Category: Lebedev, A A]]

2w11

From Proteopedia

Revision as of 08:46, 25 June 2020

Structure of the L-2-haloacid dehalogenase from Sulfolobus tokodaii

Structural highlights

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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