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2ve9

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Revision as of 07:48, 25 June 2020

Xray structure of KOPS bound gamma domain of FtsK (P. aeruginosa)

Structural highlights

2ve9 is a 10 chain structure with sequence from "bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	2iuu, 2iut, 2j5o, 2ve8
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[FTSK_PSEAE] Essential cell division protein that coordinates cell division and chromosome segregation. The N-terminus is involved in assembly of the cell-division machinery. The C-terminus functions as a DNA motor that moves dsDNA in an ATP-dependent manner towards the dif recombination site, which is located within the replication terminus region. Translocation stops specifically at Xer-dif sites, where FtsK interacts with the Xer recombinase, allowing activation of chromosome unlinking by recombination. FtsK orienting polar sequences (KOPS) guide the direction of DNA translocation. FtsK can remove proteins from DNA as it translocates, but translocation stops specifically at XerCD-dif site, thereby preventing removal of XerC and XerD from dif (Probable).^[1] ^[2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Dimeric circular chromosomes, formed by recombination between monomer sisters, cannot be segregated to daughter cells at cell division. XerCD site-specific recombination at the Escherichia coli dif site converts these dimers to monomers in a reaction that requires the DNA translocase FtsK. Short DNA sequences, KOPS (GGGNAGGG), which are polarized toward dif in the chromosome, direct FtsK translocation. FtsK interacts with KOPS through a C-terminal winged helix domain gamma. The crystal structure of three FtsKgamma domains bound to 8 bp KOPS DNA demonstrates how three gamma domains recognize KOPS. Using covalently linked dimers of FtsK, we infer that three gamma domains per hexamer are sufficient to recognize KOPS and load FtsK and subsequently activate recombination at dif. During translocation, FtsK fails to recognize an inverted KOPS sequence. Therefore, we propose that KOPS act solely as a loading site for FtsK, resulting in a unidirectionally oriented hexameric motor upon DNA.

Molecular mechanism of sequence-directed DNA loading and translocation by FtsK.,Lowe J, Ellonen A, Allen MD, Atkinson C, Sherratt DJ, Grainge I Mol Cell. 2008 Aug 22;31(4):498-509. PMID:18722176^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Massey TH, Mercogliano CP, Yates J, Sherratt DJ, Lowe J. Double-stranded DNA translocation: structure and mechanism of hexameric FtsK. Mol Cell. 2006 Aug;23(4):457-69. PMID:16916635 doi:10.1016/j.molcel.2006.06.019
↑ Lowe J, Ellonen A, Allen MD, Atkinson C, Sherratt DJ, Grainge I. Molecular mechanism of sequence-directed DNA loading and translocation by FtsK. Mol Cell. 2008 Aug 22;31(4):498-509. PMID:18722176 doi:10.1016/j.molcel.2008.05.027
↑ Lowe J, Ellonen A, Allen MD, Atkinson C, Sherratt DJ, Grainge I. Molecular mechanism of sequence-directed DNA loading and translocation by FtsK. Mol Cell. 2008 Aug 22;31(4):498-509. PMID:18722176 doi:10.1016/j.molcel.2008.05.027

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ve9"

@@ Line 1: / Line 1: @@
 ==Xray structure of KOPS bound gamma domain of FtsK (P. aeruginosa)==
-<StructureSection load='2ve9' size='340' side='right' caption='[[2ve9]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
+<StructureSection load='2ve9' size='340' side='right'caption='[[2ve9]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2ve9]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VE9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VE9 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2ve9]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VE9 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2VE9 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2iuu|2iuu]], [[2iut|2iut]], [[2j5o|2j5o]], [[2ve8|2ve8]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ve9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ve9 OCA], [http://pdbe.org/2ve9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ve9 RCSB], [http://www.ebi.ac.uk/pdbsum/2ve9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ve9 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2ve9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ve9 OCA], [http://pdbe.org/2ve9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ve9 RCSB], [http://www.ebi.ac.uk/pdbsum/2ve9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ve9 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 29: / Line 29: @@
 </div>
 <div class="pdbe-citations 2ve9" style="background-color:#fffaf0;"></div>
-==See Also==
-*[[Tubulin|Tubulin]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Allen, M D]]
 [[Category: Lowe, J]]

2ve9

From Proteopedia

Revision as of 07:48, 25 June 2020

Xray structure of KOPS bound gamma domain of FtsK (P. aeruginosa)

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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