2q80

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:2q80.jpg|left|200px]]
[[Image:2q80.jpg|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 2q80 |SIZE=350|CAPTION= <scene name='initialview01'>2q80</scene>, resolution 2.70&Aring;
+
The line below this paragraph, containing "STRUCTURE_2q80", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=GRG:GERANYLGERANYL+DIPHOSPHATE'>GRG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY=
+
or leave the SCENE parameter empty for the default display.
-
|GENE= GGPS1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
+
-->
-
|DOMAIN=
+
{{STRUCTURE_2q80| PDB=2q80 | SCENE= }}
-
|RELATEDENTRY=
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2q80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q80 OCA], [http://www.ebi.ac.uk/pdbsum/2q80 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2q80 RCSB]</span>
+
-
}}
+
'''Crystal structure of human geranylgeranyl pyrophosphate synthase bound to GGPP'''
'''Crystal structure of human geranylgeranyl pyrophosphate synthase bound to GGPP'''
Line 19: Line 16:
==About this Structure==
==About this Structure==
-
2Q80 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure supersedes the now removed PDB entry 2FVI. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q80 OCA].
+
2Q80 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2fvi 2fvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q80 OCA].
==Reference==
==Reference==
Line 36: Line 33:
[[Category: Vondelft, F.]]
[[Category: Vondelft, F.]]
[[Category: Weigelt, J.]]
[[Category: Weigelt, J.]]
-
[[Category: isopentenyl transferase]]
+
[[Category: Isopentenyl transferase]]
-
[[Category: isoprenoid pathway]]
+
[[Category: Isoprenoid pathway]]
-
[[Category: sgc]]
+
[[Category: Sgc]]
-
[[Category: structural genomic]]
+
[[Category: Structural genomic]]
-
[[Category: structural genomics consortium]]
+
[[Category: Structural genomics consortium]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 14:30:18 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:46:01 2008''
+

Revision as of 11:30, 4 May 2008

Image:2q80.jpg

Template:STRUCTURE 2q80

Crystal structure of human geranylgeranyl pyrophosphate synthase bound to GGPP


Overview

Modification of GTPases with isoprenoid molecules derived from geranylgeranyl pyrophosphate or farnesyl pyrophosphate is an essential requisite for cellular signaling pathways. The synthesis of these isoprenoids proceeds in mammals through the mevalonate pathway, and the final steps in the synthesis are catalyzed by the related enzymes farnesyl pyrophosphate synthase and geranylgeranyl pyrophosphate synthase. Both enzymes play crucial roles in cell survival, and inhibition of farnesyl pyrophosphate synthase by nitrogen-containing bisphosphonates is an established concept in the treatment of bone disorders such as osteoporosis or certain forms of cancer in bone. Here we report the crystal structure of human geranylgeranyl pyrophosphate synthase, the first mammalian ortholog to have its x-ray structure determined. It reveals that three dimers join together to form a propeller-bladed hexameric molecule with a mass of approximately 200 kDa. Structure-based sequence alignments predict this quaternary structure to be restricted to mammalian and insect orthologs, whereas fungal, bacterial, archaeal, and plant forms exhibit the dimeric organization also observed in farnesyl pyrophosphate synthase. Geranylgeranyl pyrophosphate derived from heterologous bacterial expression is tightly bound in a cavity distinct from the chain elongation site described for farnesyl pyrophosphate synthase. The structure most likely represents an inhibitory complex, which is further corroborated by steady-state kinetics, suggesting a possible feedback mechanism for regulating enzyme activity. Structural comparisons between members of this enzyme class give deeper insights into conserved features important for catalysis.

About this Structure

2Q80 is a Single protein structure of sequence from Homo sapiens. This structure supersedes the now removed PDB entry 2fvi. Full crystallographic information is available from OCA.

Reference

The crystal structure of human geranylgeranyl pyrophosphate synthase reveals a novel hexameric arrangement and inhibitory product binding., Kavanagh KL, Dunford JE, Bunkoczi G, Russell RG, Oppermann U, J Biol Chem. 2006 Aug 4;281(31):22004-12. Epub 2006 May 11. PMID:16698791 Page seeded by OCA on Sun May 4 14:30:18 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2q80"
Personal tools