2q8c

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:2q8c.jpg|left|200px]]
[[Image:2q8c.jpg|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 2q8c |SIZE=350|CAPTION= <scene name='initialview01'>2q8c</scene>, resolution 2.047&Aring;
+
The line below this paragraph, containing "STRUCTURE_2q8c", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=AKG:2-OXYGLUTARIC+ACID'>AKG</scene>, <scene name='pdbligand=M3L:N-TRIMETHYLLYSINE'>M3L</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY=
+
or leave the SCENE parameter empty for the default display.
-
|GENE= JMJD2A, JHDM3A, JMJD2, KIAA0677 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
+
-->
-
|DOMAIN=
+
{{STRUCTURE_2q8c| PDB=2q8c | SCENE= }}
-
|RELATEDENTRY=[[2q8d|2Q8D]], [[2q8e|2Q8E]]
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2q8c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q8c OCA], [http://www.ebi.ac.uk/pdbsum/2q8c PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2q8c RCSB]</span>
+
-
}}
+
'''Crystal structure of JMJD2A in ternary complex with an histone H3K9me3 peptide and 2-oxoglutarate'''
'''Crystal structure of JMJD2A in ternary complex with an histone H3K9me3 peptide and 2-oxoglutarate'''
Line 30: Line 27:
[[Category: Ortiz-Tello, P.]]
[[Category: Ortiz-Tello, P.]]
[[Category: Trievel, R C.]]
[[Category: Trievel, R C.]]
-
[[Category: histone demethylase]]
+
[[Category: Histone demethylase]]
-
[[Category: hydroxylase]]
+
[[Category: Hydroxylase]]
-
[[Category: jumonji]]
+
[[Category: Jumonji]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 14:31:40 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:46:09 2008''
+

Revision as of 11:31, 4 May 2008

Image:2q8c.jpg

Template:STRUCTURE 2q8c

Crystal structure of JMJD2A in ternary complex with an histone H3K9me3 peptide and 2-oxoglutarate


Overview

JMJD2A is a JmjC histone demethylase (HDM) that catalyzes the demethylation of di- and trimethylated Lys9 and Lys36 in histone H3 (H3K9me2/3 and H3K36me2/3). Here we present the crystal structures of the JMJD2A catalytic domain in complex with H3K9me3, H3K36me2 and H3K36me3 peptides. The structures reveal that histone substrates are recognized through a network of backbone hydrogen bonds and hydrophobic interactions that deposit the trimethyllysine into the active site. The trimethylated epsilon-ammonium cation is coordinated within a methylammonium-binding pocket through carbon-oxygen (CH...O) hydrogen bonds that position one of the zeta-methyl groups adjacent to the Fe(II) center for hydroxylation and demethylation. Mutations of the residues comprising this pocket abrogate demethylation by JMJD2A, with the exception of an S288A substitution, which augments activity, particularly toward H3K9me2. We propose that this residue modulates the methylation-state specificities of JMJD2 enzymes and other trimethyllysine-specific JmjC HDMs.

About this Structure

2Q8C is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Specificity and mechanism of JMJD2A, a trimethyllysine-specific histone demethylase., Couture JF, Collazo E, Ortiz-Tello PA, Brunzelle JS, Trievel RC, Nat Struct Mol Biol. 2007 Aug;14(8):689-95. Epub 2007 Jun 24. PMID:17589523 Page seeded by OCA on Sun May 4 14:31:40 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2q8c"
Personal tools