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2ver

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Revision as of 07:49, 25 June 2020

Structural model for the complex between the Dr adhesins and carcinoembryonic antigen (CEA)

Structural highlights

2ver is a 2 chain structure with sequence from "bacillus_coli"_migula_1895 "bacillus coli" migula 1895 and Human. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	1e07, 1ut2, 1usz, 2ixq, 1rxl
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[AFAE3_ECOLX] Hemagglutinins of uropathogenic E.coli mediate adherence to the upper urinary tract. These adhesins bind to the Dr blood group antigen and also agglutinate human erythrocytes in the presence of D-mannose (mannose-resistant hemagglutination (MRHA)). [CEAM5_HUMAN] Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Carcinoembryonic antigen (CEA)-related cell adhesion molecules (CEACAMs) are host receptors for the Dr family of adhesins of Escherichia coli. To define the mechanism for binding of Dr adhesins to CEACAM receptors, we carried out structural studies on the N-terminal domain of CEA and its complex with the Dr adhesin. The crystal structure of CEA reveals a dimer similar to other dimers formed by receptors with IgV-like domains. The structure of the CEA/Dr adhesin complex is proposed based on NMR spectroscopy and mutagenesis data in combination with biochemical characterization. The Dr adhesin/CEA interface overlaps appreciably with the region responsible for CEA dimerization. Binding kinetics, mutational analysis and spectroscopic examination of CEA dimers suggest that Dr adhesins can dissociate CEA dimers prior to the binding of monomeric forms. Our conclusions include a plausible mechanism for how E. coli, and perhaps other bacterial and viral pathogens, exploit CEACAMs. The present structure of the complex provides a powerful tool for the design of novel inhibitory strategies to treat E. coli infections.

Binding of Dr adhesins of Escherichia coli to carcinoembryonic antigen triggers receptor dissociation.,Korotkova N, Yang Y, Le Trong I, Cota E, Demeler B, Marchant J, Thomas WE, Stenkamp RE, Moseley SL, Matthews S Mol Microbiol. 2008 Jan;67(2):420-34. Epub 2007 Dec 11. PMID:18086185^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Taheri M, Saragovi U, Fuks A, Makkerh J, Mort J, Stanners CP. Self recognition in the Ig superfamily. Identification of precise subdomains in carcinoembryonic antigen required for intercellular adhesion. J Biol Chem. 2000 Sep 1;275(35):26935-43. PMID:10864933 doi:10.1074/jbc.M909242199
↑ Korotkova N, Yang Y, Le Trong I, Cota E, Demeler B, Marchant J, Thomas WE, Stenkamp RE, Moseley SL, Matthews S. Binding of Dr adhesins of Escherichia coli to carcinoembryonic antigen triggers receptor dissociation. Mol Microbiol. 2008 Jan;67(2):420-34. Epub 2007 Dec 11. PMID:18086185 doi:http://dx.doi.org/10.1111/j.1365-2958.2007.06054.x

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ver"

@@ Line 1: / Line 1: @@
 ==Structural model for the complex between the Dr adhesins and carcinoembryonic antigen (CEA)==
-<StructureSection load='2ver' size='340' side='right' caption='[[2ver]], [[NMR_Ensembles_of_Models | 1 NMR models]]' scene=''>
+<StructureSection load='2ver' size='340' side='right'caption='[[2ver]], [[NMR_Ensembles_of_Models | 1 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2ver]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895] and [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VER OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VER FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2ver]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895] and [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VER OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2VER FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MTN:S-[(1-OXYL-2,2,5,5-TETRAMETHYL-2,5-DIHYDRO-1H-PYRROL-3-YL)METHYL]+METHANESULFONOTHIOATE'>MTN</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MTN:S-[(1-OXYL-2,2,5,5-TETRAMETHYL-2,5-DIHYDRO-1H-PYRROL-3-YL)METHYL]+METHANESULFONOTHIOATE'>MTN</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1e07|1e07]], [[1ut2|1ut2]], [[1usz|1usz]], [[2ixq|2ixq]], [[1rxl|1rxl]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ver FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ver OCA], [http://pdbe.org/2ver PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ver RCSB], [http://www.ebi.ac.uk/pdbsum/2ver PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ver ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2ver FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ver OCA], [http://pdbe.org/2ver PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ver RCSB], [http://www.ebi.ac.uk/pdbsum/2ver PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ver ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 35: / Line 35: @@
 [[Category: Bacillus coli migula 1895]]
 [[Category: Human]]
+[[Category: Large Structures]]
 [[Category: Cota, E]]
 [[Category: Demeler, B]]

2ver

From Proteopedia

Revision as of 07:49, 25 June 2020

Structural model for the complex between the Dr adhesins and carcinoembryonic antigen (CEA)

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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