2qar

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:2qar.jpg|left|200px]]
[[Image:2qar.jpg|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 2qar |SIZE=350|CAPTION= <scene name='initialview01'>2qar</scene>, resolution 2.400&Aring;
+
The line below this paragraph, containing "STRUCTURE_2qar", creates the "Structure Box" on the page.
-
|SITE= <scene name='pdbsite=AC1:No3+Binding+Site+For+Residue+D+101'>AC1</scene>, <scene name='pdbsite=AC2:No3+Binding+Site+For+Residue+A+102'>AC2</scene>, <scene name='pdbsite=AC3:No3+Binding+Site+For+Residue+E+108'>AC3</scene>, <scene name='pdbsite=AC4:No3+Binding+Site+For+Residue+D+104'>AC4</scene>, <scene name='pdbsite=AC5:No3+Binding+Site+For+Residue+D+106'>AC5</scene>, <scene name='pdbsite=AC6:No3+Binding+Site+For+Residue+E+109'>AC6</scene>, <scene name='pdbsite=AC7:No3+Binding+Site+For+Residue+D+108'>AC7</scene>, <scene name='pdbsite=AC8:No3+Binding+Site+For+Residue+B+109'>AC8</scene>, <scene name='pdbsite=AC9:Nh4+Binding+Site+For+Residue+A+201'>AC9</scene> and <scene name='pdbsite=BC1:Nh4+Binding+Site+For+Residue+D+202'>BC1</scene>
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span>
+
or leave the SCENE parameter empty for the default display.
-
|GENE= E ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 Enterobacteria phage T4])
+
-->
-
|DOMAIN=
+
{{STRUCTURE_2qar| PDB=2qar | SCENE= }}
-
|RELATEDENTRY=[[2qb0|2qb0]], [[2qb1|2QB1]]
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qar FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qar OCA], [http://www.ebi.ac.uk/pdbsum/2qar PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2qar RCSB]</span>
+
-
}}
+
'''Structure of the 2TEL crystallization module fused to T4 lysozyme with a helical linker.'''
'''Structure of the 2TEL crystallization module fused to T4 lysozyme with a helical linker.'''
Line 29: Line 26:
[[Category: Bowie, J U.]]
[[Category: Bowie, J U.]]
[[Category: Nauli, S.]]
[[Category: Nauli, S.]]
-
[[Category: crystallization module]]
+
[[Category: Crystallization module]]
-
[[Category: hydrolase regulator]]
+
[[Category: Hydrolase regulator]]
-
[[Category: polymer]]
+
[[Category: Polymer]]
-
[[Category: sterile alpha motif]]
+
[[Category: Sterile alpha motif]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 14:38:52 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:47:02 2008''
+

Revision as of 11:38, 4 May 2008

Image:2qar.jpg

Template:STRUCTURE 2qar

Structure of the 2TEL crystallization module fused to T4 lysozyme with a helical linker.


Overview

Obtaining well-diffracting crystals of macromolecules remains a significant barrier to structure determination. Here we propose and test a new approach to crystallization, in which the crystallization target is fused to a polymerizing protein module, so that polymer formation drives crystallization of the target. We test the approach using a polymerization module called 2TEL, which consists of two tandem sterile alpha motif (SAM) domains from the protein translocation Ets leukemia (TEL). The 2TEL module is engineered to polymerize as the pH is lowered, which allows the subtle modulation of polymerization needed for crystal formation. We show that the 2TEL module can drive the crystallization of 11 soluble proteins, including three that resisted prior crystallization attempts. In addition, the 2TEL module crystallizes in the presence of various detergents, suggesting that it might facilitate membrane protein crystallization. The crystal structures of two fusion proteins show that the TELSAM polymer is responsible for the majority of contacts in the crystal lattice. The results suggest that biological polymers could be designed as crystallization modules.

About this Structure

2QAR is a Protein complex structure of sequences from Enterobacteria phage t4 and Escherichia coli. Full crystallographic information is available from OCA.

Reference

Polymer-driven crystallization., Nauli S, Farr S, Lee YJ, Kim HY, Faham S, Bowie JU, Protein Sci. 2007 Nov;16(11):2542-51. PMID:17962407 Page seeded by OCA on Sun May 4 14:38:52 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2qar"
Personal tools