2qf7

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[[Image:2qf7.jpg|left|200px]]
[[Image:2qf7.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2qf7 |SIZE=350|CAPTION= <scene name='initialview01'>2qf7</scene>, resolution 2.000&Aring;
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The line below this paragraph, containing "STRUCTURE_2qf7", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SAP:ADENOSINE-5&#39;-DIPHOSPHATE+MONOTHIOPHOSPHATE'>SAP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Pyruvate_carboxylase Pyruvate carboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.4.1.1 6.4.1.1] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= pyc ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=29449 Rhizobium etli])
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-->
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|DOMAIN=
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{{STRUCTURE_2qf7| PDB=2qf7 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qf7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qf7 OCA], [http://www.ebi.ac.uk/pdbsum/2qf7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2qf7 RCSB]</span>
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}}
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'''Crystal structure of a complete multifunctional pyruvate carboxylase from Rhizobium etli'''
'''Crystal structure of a complete multifunctional pyruvate carboxylase from Rhizobium etli'''
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[[Category: Rayment, I.]]
[[Category: Rayment, I.]]
[[Category: Surinya, K H.]]
[[Category: Surinya, K H.]]
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[[Category: biotin-dependent]]
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[[Category: Biotin-dependent]]
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[[Category: ligase]]
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[[Category: Ligase]]
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[[Category: multi-domain]]
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[[Category: Multi-domain]]
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[[Category: multi-functional]]
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[[Category: Multi-functional]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 14:51:08 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:48:40 2008''
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Revision as of 11:51, 4 May 2008

Image:2qf7.jpg

Template:STRUCTURE 2qf7

Crystal structure of a complete multifunctional pyruvate carboxylase from Rhizobium etli


Overview

Biotin-dependent multifunctional enzymes carry out metabolically important carboxyl group transfer reactions and are potential targets for the treatment of obesity and type 2 diabetes. These enzymes use a tethered biotin cofactor to carry an activated carboxyl group between distantly spaced active sites. The mechanism of this transfer has remained poorly understood. Here we report the complete structure of pyruvate carboxylase at 2.0 angstroms resolution, which shows its domain arrangement. The structure, when combined with mutagenic analysis, shows that intermediate transfer occurs between active sites on separate polypeptide chains. In addition, domain rearrangements associated with activator binding decrease the distance between active-site pairs, providing a mechanism for allosteric activation. This description provides insight into the function of biotin-dependent enzymes and presents a new paradigm for multifunctional enzyme catalysis.

About this Structure

2QF7 is a Single protein structure of sequence from Rhizobium etli. Full crystallographic information is available from OCA.

Reference

Domain architecture of pyruvate carboxylase, a biotin-dependent multifunctional enzyme., St Maurice M, Reinhardt L, Surinya KH, Attwood PV, Wallace JC, Cleland WW, Rayment I, Science. 2007 Aug 24;317(5841):1076-9. PMID:17717183 Page seeded by OCA on Sun May 4 14:51:08 2008

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