2qfd

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:2qfd.jpg|left|200px]]
[[Image:2qfd.jpg|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 2qfd |SIZE=350|CAPTION= <scene name='initialview01'>2qfd</scene>, resolution 2.7&Aring;
+
The line below this paragraph, containing "STRUCTURE_2qfd", creates the "Structure Box" on the page.
-
|SITE= <scene name='pdbsite=AC1:Hg+Binding+Site+For+Residue+A+1001'>AC1</scene>, <scene name='pdbsite=AC2:Hg+Binding+Site+For+Residue+B+1002'>AC2</scene>, <scene name='pdbsite=AC3:Hg+Binding+Site+For+Residue+C+1003'>AC3</scene>, <scene name='pdbsite=AC4:Hg+Binding+Site+For+Residue+D+1004'>AC4</scene>, <scene name='pdbsite=AC5:Hg+Binding+Site+For+Residue+E+1005'>AC5</scene>, <scene name='pdbsite=AC6:Hg+Binding+Site+For+Residue+F+1006'>AC6</scene>, <scene name='pdbsite=AC7:Hg+Binding+Site+For+Residue+G+1007'>AC7</scene>, <scene name='pdbsite=AC8:Hg+Binding+Site+For+Residue+H+1008'>AC8</scene>, <scene name='pdbsite=AC9:Hg+Binding+Site+For+Residue+I+1009'>AC9</scene> and <scene name='pdbsite=BC1:Hg+Binding+Site+For+Residue+J+1010'>BC1</scene>
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY=
+
or leave the SCENE parameter empty for the default display.
-
|GENE= DDX58 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
+
-->
-
|DOMAIN=
+
{{STRUCTURE_2qfd| PDB=2qfd | SCENE= }}
-
|RELATEDENTRY=[[2qfb|2QFB]]
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qfd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qfd OCA], [http://www.ebi.ac.uk/pdbsum/2qfd PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2qfd RCSB]</span>
+
-
}}
+
'''Crystal structure of the regulatory domain of human RIG-I with bound Hg'''
'''Crystal structure of the regulatory domain of human RIG-I with bound Hg'''
Line 29: Line 26:
[[Category: Lammens, A.]]
[[Category: Lammens, A.]]
[[Category: Lammens, K.]]
[[Category: Lammens, K.]]
-
[[Category: alternative splicing]]
+
[[Category: Alternative splicing]]
-
[[Category: antiviral defense]]
+
[[Category: Antiviral defense]]
-
[[Category: atp-binding]]
+
[[Category: Atp-binding]]
-
[[Category: helicase]]
+
[[Category: Helicase]]
-
[[Category: hydrolase]]
+
[[Category: Hydrolase]]
-
[[Category: immune response]]
+
[[Category: Immune response]]
-
[[Category: innate immunity]]
+
[[Category: Innate immunity]]
-
[[Category: interferon induction]]
+
[[Category: Interferon induction]]
-
[[Category: nucleotide-binding]]
+
[[Category: Nucleotide-binding]]
-
[[Category: polymorphism]]
+
[[Category: Polymorphism]]
-
[[Category: rna-binding]]
+
[[Category: Rna-binding]]
-
[[Category: ubl conjugation]]
+
[[Category: Ubl conjugation]]
-
[[Category: zinc finger]]
+
[[Category: Zinc finger]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 14:51:51 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:48:42 2008''
+

Revision as of 11:51, 4 May 2008

Image:2qfd.jpg

Template:STRUCTURE 2qfd

Crystal structure of the regulatory domain of human RIG-I with bound Hg


Overview

The ATPase RIG-I senses viral RNAs that contain 5'-triphosphates in the cytoplasm. It initiates a signaling cascade that activates innate immune response by interferon and cytokine production, providing essential antiviral protection for the host. The mode of RNA 5'-triphosphate sensing by RIG-I remains elusive. We show that the C-terminal regulatory domain RD of RIG-I binds viral RNA in a 5'-triphosphate-dependent manner and activates the RIG-I ATPase by RNA-dependent dimerization. The crystal structure of RD reveals a zinc-binding domain that is structurally related to GDP/GTP exchange factors of Rab-like GTPases. The zinc coordination site is essential for RIG-I signaling and is also conserved in MDA5 and LGP2, suggesting related RD domains in all three enzymes. Structure-guided mutagenesis identifies a positively charged groove as likely 5'-triphosphate-binding site of RIG-I. This groove is distinct in MDA5 and LGP2, raising the possibility that RD confers ligand specificity.

About this Structure

2QFD is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

The C-Terminal Regulatory Domain Is the RNA 5'-Triphosphate Sensor of RIG-I., Cui S, Eisenacher K, Kirchhofer A, Brzozka K, Lammens A, Lammens K, Fujita T, Conzelmann KK, Krug A, Hopfner KP, Mol Cell. 2008 Feb 1;29(2):169-179. PMID:18243112 Page seeded by OCA on Sun May 4 14:51:51 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2qfd"
Personal tools