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2qfb

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[[Image:2qfb.jpg|left|200px]]
[[Image:2qfb.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2qfb |SIZE=350|CAPTION= <scene name='initialview01'>2qfb</scene>, resolution 3.0&Aring;
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The line below this paragraph, containing "STRUCTURE_2qfb", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:Zn+Binding+Site+For+Residue+A+1001'>AC1</scene>, <scene name='pdbsite=AC2:Zn+Binding+Site+For+Residue+B+1002'>AC2</scene>, <scene name='pdbsite=AC3:Zn+Binding+Site+For+Residue+C+1003'>AC3</scene>, <scene name='pdbsite=AC4:Zn+Binding+Site+For+Residue+D+1004'>AC4</scene>, <scene name='pdbsite=AC5:Zn+Binding+Site+For+Residue+E+1005'>AC5</scene>, <scene name='pdbsite=AC6:Zn+Binding+Site+For+Residue+F+1006'>AC6</scene>, <scene name='pdbsite=AC7:Zn+Binding+Site+For+Residue+G+1007'>AC7</scene>, <scene name='pdbsite=AC8:Zn+Binding+Site+For+Residue+H+1008'>AC8</scene>, <scene name='pdbsite=AC9:Zn+Binding+Site+For+Residue+I+1009'>AC9</scene> and <scene name='pdbsite=BC1:Zn+Binding+Site+For+Residue+J+1010'>BC1</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= DDX58 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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-->
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|DOMAIN=
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{{STRUCTURE_2qfb| PDB=2qfb | SCENE= }}
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|RELATEDENTRY=[[2qfd|2QFD]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qfb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qfb OCA], [http://www.ebi.ac.uk/pdbsum/2qfb PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2qfb RCSB]</span>
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}}
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'''Crystal structure of the regulatory domain of human RIG-I with bound Zn'''
'''Crystal structure of the regulatory domain of human RIG-I with bound Zn'''
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[[Category: Lammens, A.]]
[[Category: Lammens, A.]]
[[Category: Lammens, K.]]
[[Category: Lammens, K.]]
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[[Category: alternative splicing]]
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[[Category: Alternative splicing]]
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[[Category: antiviral defense]]
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[[Category: Antiviral defense]]
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[[Category: atp-binding]]
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[[Category: Atp-binding]]
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[[Category: helicase]]
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[[Category: Helicase]]
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[[Category: hydrolase]]
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[[Category: Hydrolase]]
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[[Category: immune response]]
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[[Category: Immune response]]
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[[Category: innate immunity]]
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[[Category: Innate immunity]]
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[[Category: interferon induction]]
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[[Category: Interferon induction]]
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[[Category: nucleotide-binding]]
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[[Category: Nucleotide-binding]]
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[[Category: polymorphism]]
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[[Category: Polymorphism]]
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[[Category: rna-binding]]
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[[Category: Rna-binding]]
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[[Category: ubl conjugation]]
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[[Category: Ubl conjugation]]
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[[Category: zinc finger]]
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[[Category: Zinc finger]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 14:51:28 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:48:38 2008''
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Revision as of 11:51, 4 May 2008

Image:2qfb.jpg

Template:STRUCTURE 2qfb

Crystal structure of the regulatory domain of human RIG-I with bound Zn


Overview

The ATPase RIG-I senses viral RNAs that contain 5'-triphosphates in the cytoplasm. It initiates a signaling cascade that activates innate immune response by interferon and cytokine production, providing essential antiviral protection for the host. The mode of RNA 5'-triphosphate sensing by RIG-I remains elusive. We show that the C-terminal regulatory domain RD of RIG-I binds viral RNA in a 5'-triphosphate-dependent manner and activates the RIG-I ATPase by RNA-dependent dimerization. The crystal structure of RD reveals a zinc-binding domain that is structurally related to GDP/GTP exchange factors of Rab-like GTPases. The zinc coordination site is essential for RIG-I signaling and is also conserved in MDA5 and LGP2, suggesting related RD domains in all three enzymes. Structure-guided mutagenesis identifies a positively charged groove as likely 5'-triphosphate-binding site of RIG-I. This groove is distinct in MDA5 and LGP2, raising the possibility that RD confers ligand specificity.

About this Structure

2QFB is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

The C-Terminal Regulatory Domain Is the RNA 5'-Triphosphate Sensor of RIG-I., Cui S, Eisenacher K, Kirchhofer A, Brzozka K, Lammens A, Lammens K, Fujita T, Conzelmann KK, Krug A, Hopfner KP, Mol Cell. 2008 Feb 1;29(2):169-179. PMID:18243112 Page seeded by OCA on Sun May 4 14:51:28 2008

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