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2w83

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Revision as of 08:19, 1 July 2020

Crystal structure of the ARF6 GTPase in complex with a specific effector, JIP4

Structural highlights

2w83 is a 5 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, , ,
Related:	2a5f, 1e0s, 2a5d, 2a5g, 2j5x
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The JNK-interacting proteins, JIP3 and JIP4, are specific effectors of the small GTP-binding protein ARF6. The interaction of ARF6-GTP with the second leucine zipper (LZII) domains of JIP3/JIP4 regulates the binding of JIPs to kinesin-1 and dynactin. Here, we report the crystal structure of ARF6-GTP bound to the JIP4-LZII at 1.9 A resolution. The complex is a heterotetramer with dyad symmetry arranged in an ARF6-(JIP4)(2)-ARF6 configuration. Comparison of the ARF6-JIP4 interface with the equivalent region of ARF1 shows the structural basis of JIP4's specificity for ARF6. Using site-directed mutagenesis and surface plasmon resonance, we further show that non-conserved residues at the switch region borders are the key structural determinants of JIP4 specificity. A structure-derived model of the association of the ARF6-JIP3/JIP4 complex with membranes shows that the JIP4-LZII coiled-coil should lie along the membrane to prevent steric hindrances, resulting in only one ARF6 molecule bound. Such a heterotrimeric complex gives insights to better understand the ARF6-mediated motor switch regulatory function.

The structural basis of Arf effector specificity: the crystal structure of ARF6 in a complex with JIP4.,Isabet T, Montagnac G, Regazzoni K, Raynal B, El Khadali F, England P, Franco M, Chavrier P, Houdusse A, Menetrey J EMBO J. 2009 Sep 16;28(18):2835-45. Epub 2009 Jul 30. PMID:19644450^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Isabet T, Montagnac G, Regazzoni K, Raynal B, El Khadali F, England P, Franco M, Chavrier P, Houdusse A, Menetrey J. The structural basis of Arf effector specificity: the crystal structure of ARF6 in a complex with JIP4. EMBO J. 2009 Sep 16;28(18):2835-45. Epub 2009 Jul 30. PMID:19644450 doi:10.1038/emboj.2009.209

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2w83"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the ARF6 GTPase in complex with a specific effector, JIP4==
-<StructureSection load='2w83' size='340' side='right' caption='[[2w83]], [[Resolution|resolution]] 1.93&Aring;' scene=''>
+<StructureSection load='2w83' size='340' side='right'caption='[[2w83]], [[Resolution|resolution]] 1.93&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2w83]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W83 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2W83 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2w83]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W83 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2W83 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2a5f|2a5f]], [[1e0s|1e0s]], [[2a5d|2a5d]], [[2a5g|2a5g]], [[2j5x|2j5x]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w83 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w83 OCA], [http://pdbe.org/2w83 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2w83 RCSB], [http://www.ebi.ac.uk/pdbsum/2w83 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2w83 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2w83 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w83 OCA], [http://pdbe.org/2w83 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2w83 RCSB], [http://www.ebi.ac.uk/pdbsum/2w83 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2w83 ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 32: / Line 32: @@
 </StructureSection>
 [[Category: Human]]
+[[Category: Large Structures]]
 [[Category: Chavrier, P]]
 [[Category: England, P]]

2w83

From Proteopedia

Revision as of 08:19, 1 July 2020

Crystal structure of the ARF6 GTPase in complex with a specific effector, JIP4

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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