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2w6r

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Revision as of 08:16, 1 July 2020

Crystal structure of an artificial (ba)8-barrel protein designed from identical half barrels

Structural highlights

2w6r is a 1 chain structure with sequence from Atcc 43589. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	2a0n, 1thf, 1gpw, 1vh7
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[HIS6_THEMA] IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the HisH subunit.[HAMAP-Rule:MF_01013]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Ample evidence suggests that the ubiquitous (betaalpha)(8)-barrel enzyme fold has evolved by the duplication and fusion of an ancestral (betaalpha)(4)-half-barrel. To reconstruct this process in the laboratory with a model protein, we earlier fused two copies of the C-terminal half-barrel HisF-C of imidazole glycerol phosphate synthase (HisF) and stepwise stabilized the resulting HisF-CC construct. We now further increased its stability and solubility by introducing two additional amino acid exchanges, which allowed us to crystallize the resulting artificial (betaalpha)(8)-barrel protein HisF-C***C. The analysis of its X-ray structure at 2.1 A resolution reveals a striking similarity to wild-type HisF, helps us to understand its improved stability, and provides further insights into the evolution of (betaalpha)(8)-barrel proteins.

High-Resolution Crystal Structure of an Artificial (betaalpha)(8)-Barrel Protein Designed from Identical Half-Barrels (dagger) (double dagger).,Hocker B, Lochner A, Seitz T, Claren J, Sterner R Biochemistry. 2009 Jan 23. PMID:19166324^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hocker B, Lochner A, Seitz T, Claren J, Sterner R. High-Resolution Crystal Structure of an Artificial (betaalpha)(8)-Barrel Protein Designed from Identical Half-Barrels (dagger) (double dagger). Biochemistry. 2009 Jan 23. PMID:19166324 doi:10.1021/bi802125b

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2w6r"

@@ Line 1: / Line 1: @@
 ==Crystal structure of an artificial (ba)8-barrel protein designed from identical half barrels==
-<StructureSection load='2w6r' size='340' side='right' caption='[[2w6r]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
+<StructureSection load='2w6r' size='340' side='right'caption='[[2w6r]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2w6r]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43589 Atcc 43589]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W6R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2W6R FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2w6r]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43589 Atcc 43589]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W6R OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2W6R FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2a0n|2a0n]], [[1thf|1thf]], [[1gpw|1gpw]], [[1vh7|1vh7]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w6r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w6r OCA], [http://pdbe.org/2w6r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2w6r RCSB], [http://www.ebi.ac.uk/pdbsum/2w6r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2w6r ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2w6r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w6r OCA], [http://pdbe.org/2w6r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2w6r RCSB], [http://www.ebi.ac.uk/pdbsum/2w6r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2w6r ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 33: / Line 33: @@
 </StructureSection>
 [[Category: Atcc 43589]]
+[[Category: Large Structures]]
 [[Category: Claren, J]]
 [[Category: Hocker, B]]

2w6r

From Proteopedia

Revision as of 08:16, 1 July 2020

Crystal structure of an artificial (ba)8-barrel protein designed from identical half barrels

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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