2qiy

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[[Image:2qiy.gif|left|200px]]
[[Image:2qiy.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2qiy |SIZE=350|CAPTION= <scene name='initialview01'>2qiy</scene>, resolution 1.69&Aring;
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The line below this paragraph, containing "STRUCTURE_2qiy", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND=
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin_thiolesterase Ubiquitin thiolesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.2.15 3.1.2.15] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= BRE5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), UBP3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
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-->
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|DOMAIN=
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{{STRUCTURE_2qiy| PDB=2qiy | SCENE= }}
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|RELATEDENTRY=[[1zx2|1ZX2]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qiy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qiy OCA], [http://www.ebi.ac.uk/pdbsum/2qiy PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2qiy RCSB]</span>
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}}
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'''yeast Deubiquitinase Ubp3 and Bre5 cofactor complex'''
'''yeast Deubiquitinase Ubp3 and Bre5 cofactor complex'''
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[[Category: Liu, X.]]
[[Category: Liu, X.]]
[[Category: Marmorstein, R.]]
[[Category: Marmorstein, R.]]
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[[Category: deubiquitylation]]
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[[Category: Deubiquitylation]]
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[[Category: hydrolase]]
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[[Category: Hydrolase]]
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[[Category: ntf2]]
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[[Category: Ntf2]]
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[[Category: phosphorylation]]
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[[Category: Phosphorylation]]
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[[Category: protein-protein recognition]]
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[[Category: Protein-protein recognition]]
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[[Category: rna-binding]]
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[[Category: Rna-binding]]
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[[Category: signaling protein/hydrolase complex]]
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[[Category: Signaling protein/hydrolase complex]]
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[[Category: thiol protease]]
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[[Category: Thiol protease]]
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[[Category: ubiquitin-specific processing proteases(ubps)]]
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[[Category: Ubl conjugation pathway]]
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[[Category: ubl conjugation pathway]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 15:02:07 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:49:47 2008''
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Revision as of 12:02, 4 May 2008

Image:2qiy.gif

Template:STRUCTURE 2qiy

yeast Deubiquitinase Ubp3 and Bre5 cofactor complex


Overview

Yeast Ubp3 and its co-factor Bre5 form a deubiquitylation complex to regulate protein transport between the endoplasmic reticulum and Golgi compartments of the cell. A novel N-terminal domain of the Ubp3 catalytic subunit forms a complex with the NTF2-like domain of the Bre5 regulatory subunit. Here, we report the X-ray crystal structure of an Ubp3-Bre5 complex and show that it forms a symmetric hetero-tetrameric complex in which the Bre5 NTF2-like domain dimer interacts with two L-shaped beta-strand-turn-alpha-helix motifs of Ubp3. The Ubp3 N-terminal domain binds within a hydrophobic cavity on the surface of the Bre5 NTF2-like domain subunit with conserved residues within both proteins interacting predominantly through antiparallel beta-sheet hydrogen bonds and van der Waals contacts. Structure-based mutagenesis and functional studies confirm the significance of the observed interactions for Ubp3-Bre5 association in vitro and Ubp3 function in vivo. Comparison of the structure to other protein complexes with NTF2-like domains shows that the Ubp3-Bre5 interface is novel. Together, these studies provide new insights into Ubp3 recognition by Bre5 and into protein recognition by NTF2-like domains.

About this Structure

2QIY is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Molecular basis for bre5 cofactor recognition by the ubp3 deubiquitylating enzyme., Li K, Ossareh-Nazari B, Liu X, Dargemont C, Marmorstein R, J Mol Biol. 2007 Sep 7;372(1):194-204. Epub 2007 Jun 27. PMID:17632125 Page seeded by OCA on Sun May 4 15:02:07 2008

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