2bt6
From Proteopedia
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| - | [[Image:2bt6.gif|left|200px]]<br /> | + | [[Image:2bt6.gif|left|200px]]<br /><applet load="2bt6" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2bt6" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="2bt6, resolution 1.50Å" /> | caption="2bt6, resolution 1.50Å" /> | ||
'''RU(BPY)2(MBPY)-MODIFIED BOVINE ADRENODOXIN'''<br /> | '''RU(BPY)2(MBPY)-MODIFIED BOVINE ADRENODOXIN'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2BT6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with MG, FES and RUA as [http://en.wikipedia.org/wiki/ligands ligands]. | + | 2BT6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with MG, FES and RUA as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Mg Binding Site For Chain B'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BT6 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: transit peptide]] | [[Category: transit peptide]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 18:57:54 2007'' |
Revision as of 16:48, 18 December 2007
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RU(BPY)2(MBPY)-MODIFIED BOVINE ADRENODOXIN
Overview
Bovine adrenodoxin (Adx) plays an important role in the electron-transfer, process in the mitochondrial steroid hydroxylase system of the bovine, adrenal cortex. Using electron paramagnetic resonance (EPR) spectroscopy, we showed that photoreduction of the [2Fe-2S] cluster of Adx via, (4'-methyl-2,2'-bipyridine)bis(2,2'-bipyridine)ruthenium(II), [Ru(bpy)2(mbpy)] covalently attached to the protein surface can be used as, a new approach to probe the "shuttle" hypothesis for the electron transfer, by Adx. The 1.5 A resolution crystal structure of a 1:1, Ru(bpy)2(mbpy)-Adx(1-108) complex reveals the site of modification, Cys95, and allows to predict the possible intramolecular electron-transfer, pathways within the complex. Photoreduction of uncoupled Adx, mutant, Adx(1-108), and Ru(bpy)2(mbpy)-Adx(1-108) using safranin T as the, mediating electron donor suggests that two electrons are transferred from, the dye to Adx. The intramolecular photoreduction rate constant for the, ruthenated Adx has been determined and is discussed according to the, predicted pathways.
About this Structure
2BT6 is a Single protein structure of sequence from Bos taurus with MG, FES and RUA as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Light-induced reduction of bovine adrenodoxin via the covalently bound ruthenium(II) bipyridyl complex: intramolecular electron transfer and crystal structure., Halavaty A, Muller JJ, Contzen J, Jung C, Hannemann F, Bernhardt R, Galander M, Lendzian F, Heinemann U, Biochemistry. 2006 Jan 24;45(3):709-18. PMID:16411746
Page seeded by OCA on Tue Dec 18 18:57:54 2007
Categories: Bos taurus | Single protein | Bernhardt, R. | Contzen, J. | Galander, M. | Halavaty, A. | Hannemann, F. | Heinemann, U. | Jung, C. | Lendzian, F. | Mueller, J.J. | FES | MG | RUA | 2fe-2s | Bovine adrenodoxin | Electron transport | Intramolecular electron transfer | Iron | Iron-sulfur | Metal-binding | Mitochondrion | Ruthenium(ii) bipyridyl complex | Transit peptide
