2qo3

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[[Image:2qo3.jpg|left|200px]]
[[Image:2qo3.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2qo3 |SIZE=350|CAPTION= <scene name='initialview01'>2qo3</scene>, resolution 2.59&Aring;
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The line below this paragraph, containing "STRUCTURE_2qo3", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CER:(2S,+3R)-3-HYDROXY-4-OXO-7,10-TRANS,TRANS-DODECADIENAMIDE'>CER</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Erythronolide_synthase Erythronolide synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.94 2.3.1.94] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= eryAII ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1836 Saccharopolyspora erythraea])
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-->
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|DOMAIN=
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{{STRUCTURE_2qo3| PDB=2qo3 | SCENE= }}
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|RELATEDENTRY=[[2hg4|2HG4]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qo3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qo3 OCA], [http://www.ebi.ac.uk/pdbsum/2qo3 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2qo3 RCSB]</span>
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}}
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'''Crystal Structure of [KS3][AT3] didomain from module 3 of 6-deoxyerthronolide B synthase'''
'''Crystal Structure of [KS3][AT3] didomain from module 3 of 6-deoxyerthronolide B synthase'''
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[[Category: Kim, C Y.]]
[[Category: Kim, C Y.]]
[[Category: Tang, Y.]]
[[Category: Tang, Y.]]
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[[Category: acyltransferase]]
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[[Category: Acyltransferase]]
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[[Category: ketosynthase]]
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[[Category: Ketosynthase]]
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[[Category: phosphopantetheine]]
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[[Category: Phosphopantetheine]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 15:16:55 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:51:18 2008''
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Revision as of 12:16, 4 May 2008

Image:2qo3.jpg

Template:STRUCTURE 2qo3

Crystal Structure of [KS3][AT3] didomain from module 3 of 6-deoxyerthronolide B synthase


Overview

We report the 2.6 A X-ray crystal structure of a 190 kDa homodimeric fragment from module 3 of the 6-deoxyerthronolide B synthase covalently bound to the inhibitor cerulenin. The structure shows two well-organized interdomain linker regions in addition to the full-length ketosynthase (KS) and acyltransferase (AT) domains. Analysis of the substrate-binding site of the KS domain suggests that a loop region at the homodimer interface influences KS substrate specificity. We also describe a model for the interaction of the catalytic domains with the acyl carrier protein (ACP) domain. The ACP is proposed to dock within a deep cleft between the KS and AT domains, with interactions that span both the KS homodimer and AT domain. In conjunction with other recent data, our results provide atomic resolution pictures of several catalytically relevant protein interactions in this remarkable family of modular megasynthases.

About this Structure

2QO3 is a Single protein structure of sequence from Saccharopolyspora erythraea. Full crystallographic information is available from OCA.

Reference

Structural and mechanistic analysis of protein interactions in module 3 of the 6-deoxyerythronolide B synthase., Tang Y, Chen AY, Kim CY, Cane DE, Khosla C, Chem Biol. 2007 Aug;14(8):931-43. PMID:17719492 Page seeded by OCA on Sun May 4 15:16:55 2008

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