1qmq

From Proteopedia

(Difference between revisions)

Revision as of 13:11, 16 December 2020

Optical detection of cytochrome P450 by sensitizer-linked substrates

Structural highlights

1qmq is a 1 chain structure with sequence from "bacillus_fluorescens_putidus"_flugge_1886 "bacillus fluorescens putidus" flugge 1886. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
Related:	1dz9, 5cp4, 1pha, 2h7q, 1dz6, 1t88, 2cpp, 1p2y, 6cpp, 5cpp, 1iwk, 4cp4, 2a1m, 1j51, 1geb, 2fe6, 1rf9, 1t87, 1akd, 2h7r, 1dz4, 8cpp, 1cp4, 4cpp, 1phc, 1o76, 2h7s, 1phb, 1iwi, 6cp4, 1c8j, 1phd, 1phg, 1noo, 1uyu, 7cpp, 1gjm, 1dz8, 2feu, 1t86, 2cp4, 1t85, 1lwl, 1phf, 1yrd, 1k2o, 1gek, 3cpp, 1re9, 1phe, 2a1o, 2a1n, 2fer, 1p7r, 1iwj, 1gem, 3cp4, 1yrc, 1mpw
Gene:	CYP101 ("Bacillus fluorescens putidus" Flugge 1886)
Activity:	Camphor 5-monooxygenase, with EC number 1.14.15.1
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CPXA_PSEPU] Involved in a camphor oxidation system.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The ability to detect, characterize, and manipulate specific biomolecules in complex media is critical for understanding metabolic processes. Particularly important targets are oxygenases (cytochromes P450) involved in drug metabolism and many disease states, including liver and kidney dysfunction, neurological disorders, and cancer. We have found that Ru photosensitizers linked to P450 substrates specifically recognize submicromolar cytochrome P450(cam) in the presence of other heme proteins. In the P450:Ru-substrate conjugates, energy transfer to the heme dramatically accelerates the Ru-luminescence decay. The crystal structure of a P450(cam):Ru-adamantyl complex reveals access to the active center via a channel whose depth (Ru-Fe distance is 21 A) is virtually the same as that extracted from an analysis of the energy-transfer kinetics. Suitably constructed libraries of sensitizer-linked substrates could be employed to probe the steric and electronic properties of buried active sites.

Optical detection of cytochrome P450 by sensitizer-linked substrates.,Dmochowski IJ, Crane BR, Wilker JJ, Winkler JR, Gray HB Proc Natl Acad Sci U S A. 1999 Nov 9;96(23):12987-90. PMID:10557259^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Dmochowski IJ, Crane BR, Wilker JJ, Winkler JR, Gray HB. Optical detection of cytochrome P450 by sensitizer-linked substrates. Proc Natl Acad Sci U S A. 1999 Nov 9;96(23):12987-90. PMID:10557259

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1qmq"

@@ Line 1: / Line 1: @@
 ==Optical detection of cytochrome P450 by sensitizer-linked substrates==
-<StructureSection load='1qmq' size='340' side='right' caption='[[1qmq]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
+<StructureSection load='1qmq' size='340' side='right'caption='[[1qmq]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1qmq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_fluorescens_putidus"_flugge_1886 "bacillus fluorescens putidus" flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QMQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QMQ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1qmq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_fluorescens_putidus"_flugge_1886 "bacillus fluorescens putidus" flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QMQ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1QMQ FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=DRB:DELTA-BIS(2,2-BIPYRIDINE)-(5-METHYL-2-2-BIPYRIDINE)-C9-ADAMANTANE+RUTHENIUM+(II)'>DRB</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=DRB:DELTA-BIS(2,2-BIPYRIDINE)-(5-METHYL-2-2-BIPYRIDINE)-C9-ADAMANTANE+RUTHENIUM+(II)'>DRB</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1dz9|1dz9]], [[5cp4|5cp4]], [[1pha|1pha]], [[2h7q|2h7q]], [[1dz6|1dz6]], [[1t88|1t88]], [[2cpp|2cpp]], [[1p2y|1p2y]], [[6cpp|6cpp]], [[5cpp|5cpp]], [[1iwk|1iwk]], [[4cp4|4cp4]], [[2a1m|2a1m]], [[1j51|1j51]], [[1geb|1geb]], [[2fe6|2fe6]], [[1rf9|1rf9]], [[1t87|1t87]], [[1akd|1akd]], [[2h7r|2h7r]], [[1dz4|1dz4]], [[8cpp|8cpp]], [[1cp4|1cp4]], [[4cpp|4cpp]], [[1phc|1phc]], [[1o76|1o76]], [[2h7s|2h7s]], [[1phb|1phb]], [[1iwi|1iwi]], [[6cp4|6cp4]], [[1c8j|1c8j]], [[1phd|1phd]], [[1phg|1phg]], [[1noo|1noo]], [[1uyu|1uyu]], [[7cpp|7cpp]], [[1gjm|1gjm]], [[1dz8|1dz8]], [[2feu|2feu]], [[1t86|1t86]], [[2cp4|2cp4]], [[1t85|1t85]], [[1lwl|1lwl]], [[1phf|1phf]], [[1yrd|1yrd]], [[1k2o|1k2o]], [[1gek|1gek]], [[3cpp|3cpp]], [[1re9|1re9]], [[1phe|1phe]], [[2a1o|2a1o]], [[2a1n|2a1n]], [[2fer|2fer]], [[1p7r|1p7r]], [[1iwj|1iwj]], [[1gem|1gem]], [[3cp4|3cp4]], [[1yrc|1yrc]], [[1mpw|1mpw]]</td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1dz9|1dz9]], [[5cp4|5cp4]], [[1pha|1pha]], [[2h7q|2h7q]], [[1dz6|1dz6]], [[1t88|1t88]], [[2cpp|2cpp]], [[1p2y|1p2y]], [[6cpp|6cpp]], [[5cpp|5cpp]], [[1iwk|1iwk]], [[4cp4|4cp4]], [[2a1m|2a1m]], [[1j51|1j51]], [[1geb|1geb]], [[2fe6|2fe6]], [[1rf9|1rf9]], [[1t87|1t87]], [[1akd|1akd]], [[2h7r|2h7r]], [[1dz4|1dz4]], [[8cpp|8cpp]], [[1cp4|1cp4]], [[4cpp|4cpp]], [[1phc|1phc]], [[1o76|1o76]], [[2h7s|2h7s]], [[1phb|1phb]], [[1iwi|1iwi]], [[6cp4|6cp4]], [[1c8j|1c8j]], [[1phd|1phd]], [[1phg|1phg]], [[1noo|1noo]], [[1uyu|1uyu]], [[7cpp|7cpp]], [[1gjm|1gjm]], [[1dz8|1dz8]], [[2feu|2feu]], [[1t86|1t86]], [[2cp4|2cp4]], [[1t85|1t85]], [[1lwl|1lwl]], [[1phf|1phf]], [[1yrd|1yrd]], [[1k2o|1k2o]], [[1gek|1gek]], [[3cpp|3cpp]], [[1re9|1re9]], [[1phe|1phe]], [[2a1o|2a1o]], [[2a1n|2a1n]], [[2fer|2fer]], [[1p7r|1p7r]], [[1iwj|1iwj]], [[1gem|1gem]], [[3cp4|3cp4]], [[1yrc|1yrc]], [[1mpw|1mpw]]</div></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CYP101 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=303 "Bacillus fluorescens putidus" Flugge 1886])</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Camphor_5-monooxygenase Camphor 5-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.15.1 1.14.15.1] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qmq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qmq OCA], [http://pdbe.org/1qmq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1qmq RCSB], [http://www.ebi.ac.uk/pdbsum/1qmq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1qmq ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1qmq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qmq OCA], [http://pdbe.org/1qmq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1qmq RCSB], [http://www.ebi.ac.uk/pdbsum/1qmq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1qmq ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 33: / Line 33: @@
 ==See Also==
-*[[Cytochrome P450|Cytochrome P450]]
+*[[Cytochrome P450 3D structures|Cytochrome P450 3D structures]]
 == References ==
 <references/>
@@ Line 40: / Line 40: @@
 [[Category: Bacillus fluorescens putidus flugge 1886]]
 [[Category: Camphor 5-monooxygenase]]
+[[Category: Large Structures]]
 [[Category: Crane, B R]]
 [[Category: Dmochowski, I J]]

1qmq

From Proteopedia

Revision as of 13:11, 16 December 2020

Optical detection of cytochrome P450 by sensitizer-linked substrates

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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