2qow

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[[Image:2qow.gif|left|200px]]
[[Image:2qow.gif|left|200px]]
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{{Structure
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|PDB= 2qow |SIZE=350|CAPTION= <scene name='initialview01'>2qow</scene>, resolution 3.93&Aring;
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The line below this paragraph, containing "STRUCTURE_2qow", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SCM:SPECTINOMYCIN'>SCM</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
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{{STRUCTURE_2qow| PDB=2qow | SCENE= }}
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|RELATEDENTRY=[[2qou|2QOU]], [[2qov|2QOV]], [[2qox|2QOX]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qow FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qow OCA], [http://www.ebi.ac.uk/pdbsum/2qow PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2qow RCSB]</span>
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'''Crystal structure of the bacterial ribosome from Escherichia coli in complex with spectinomycin. This file contains the 30S subunit of the second 70S ribosome, with spectinomycin bound. The entire crystal structure contains two 70S ribosomes.'''
'''Crystal structure of the bacterial ribosome from Escherichia coli in complex with spectinomycin. This file contains the 30S subunit of the second 70S ribosome, with spectinomycin bound. The entire crystal structure contains two 70S ribosomes.'''
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[[Category: Holton, J M.]]
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[[Category: Shoji, S.]]
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[[Category: antibiotic resistance]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 15:20:38 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:51:35 2008''
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Revision as of 12:20, 4 May 2008

Image:2qow.gif

Template:STRUCTURE 2qow

Crystal structure of the bacterial ribosome from Escherichia coli in complex with spectinomycin. This file contains the 30S subunit of the second 70S ribosome, with spectinomycin bound. The entire crystal structure contains two 70S ribosomes.


Overview

The widely used antibiotic spectinomycin inhibits bacterial protein synthesis by blocking translocation of messenger RNA and transfer RNAs on the ribosome. Here, we show that in crystals of the Escherichia coli 70S ribosome spectinomycin binding traps a distinct swiveling state of the head domain of the small ribosomal subunit. Spectinomycin also alters the rate and completeness of reverse translocation in vitro. These structural and biochemical data indicate that in solution spectinomycin sterically blocks swiveling of the head domain of the small ribosomal subunit and thereby disrupts the translocation cycle.

About this Structure

2QOW is a Protein complex structure of sequences from Escherichia coli and Salmonella typhimurium. Full crystallographic information is available from OCA.

Reference

A steric block in translation caused by the antibiotic spectinomycin., Borovinskaya MA, Shoji S, Holton JM, Fredrick K, Cate JH, ACS Chem Biol. 2007 Aug 17;2(8):545-52. Epub 2007 Aug 10. PMID:17696316 Page seeded by OCA on Sun May 4 15:20:38 2008

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