This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

2x51

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 15:05, 8 July 2020

M6 delta Insert1

Structural highlights

2x51 is a 2 chain structure with sequence from Drome and Pig. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
Related:	2v26, 2vas, 2bbm, 4cln, 1mxe, 2bkh, 2vb6, 2bki, 2bbn, 3l9i
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[MYO6_PIG] Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Myosin 6 is a reverse-direction motor protein that moves towards the minus-end of actin filaments. Has slow rate of actin-activated ADP release due to weak ATP binding. Functions in a variety of intracellular processes such as vesicular membrane trafficking and cell migration. Required for the structural integrity of the Golgi apparatus via the p53-dependent pro-survival pathway. Appears to be involved in a very early step of clathrin-mediated endocytosis in polarized epithelial cells. May act as a regulator of F-actin dynamics. May play a role in transporting DAB2 from the plasma membrane to specific cellular targets. Required for structural integrity of inner ear hair cells (By similarity).^[1] [CALM_DROME] Calmodulin mediates the control of a large number of enzymes, ion channels and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases.

Publication Abstract from PubMed

Myosin VI is unique in its directionality among myosin superfamily members and also displays a slow and strain-dependent rate of ATP binding that allows for gating between its heads. In this study we demonstrate that leucine 310 is positioned by a class VI-specific insert, insert-1, so as to account for the selective hindrance of ATP versus ADP binding. Mutation of leucine 310 to glycine removes all influence of insert-1 on ATP binding. Furthermore, by analyzing myosin VI structures with either leucine 310 substituted to a glycine or complete removal of insert-1, we conclude that nucleotides may initially bind to myosin by their purine rings before docking their phosphate moieties. Otherwise, insert-1 could not exert a differential influence on ATP versus ADP binding.

Role of Insert-1 of Myosin VI in Modulating Nucleotide Affinity.,Pylypenko O, Song L, Squires G, Liu X, Zong AB, Houdusse A, Sweeney HL J Biol Chem. 2011 Apr 1;286(13):11716-23. Epub 2011 Jan 29. PMID:21278381^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Naccache SN, Hasson T. Myosin VI altered at threonine 406 stabilizes actin filaments in vivo. Cell Motil Cytoskeleton. 2006 Oct;63(10):633-45. PMID:16917816 doi:http://dx.doi.org/10.1002/cm.20150
↑ Pylypenko O, Song L, Squires G, Liu X, Zong AB, Houdusse A, Sweeney HL. Role of Insert-1 of Myosin VI in Modulating Nucleotide Affinity. J Biol Chem. 2011 Apr 1;286(13):11716-23. Epub 2011 Jan 29. PMID:21278381 doi:10.1074/jbc.M110.200626

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2x51"

@@ Line 1: / Line 1: @@
 ==M6 delta Insert1==
-<StructureSection load='2x51' size='340' side='right' caption='[[2x51]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
+<StructureSection load='2x51' size='340' side='right'caption='[[2x51]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2x51]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome] and [http://en.wikipedia.org/wiki/Pig Pig]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X51 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2X51 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2x51]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome] and [http://en.wikipedia.org/wiki/Pig Pig]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X51 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2X51 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2v26|2v26]], [[2vas|2vas]], [[2bbm|2bbm]], [[4cln|4cln]], [[1mxe|1mxe]], [[2bkh|2bkh]], [[2vb6|2vb6]], [[2bki|2bki]], [[2bbn|2bbn]], [[3l9i|3l9i]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2x51 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x51 OCA], [http://pdbe.org/2x51 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2x51 RCSB], [http://www.ebi.ac.uk/pdbsum/2x51 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2x51 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2x51 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x51 OCA], [http://pdbe.org/2x51 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2x51 RCSB], [http://www.ebi.ac.uk/pdbsum/2x51 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2x51 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 21: / Line 21: @@
 ==See Also==
-*[[Calmodulin|Calmodulin]]
+*[[Calmodulin 3D structures|Calmodulin 3D structures]]
-*[[Myosin|Myosin]]
+*[[Myosin 3D Structures|Myosin 3D Structures]]
 == References ==
 <references/>
@@ Line 28: / Line 28: @@
 </StructureSection>
 [[Category: Drome]]
+[[Category: Large Structures]]
 [[Category: Pig]]
 [[Category: Houdusse, A]]

2x51

From Proteopedia

Revision as of 15:05, 8 July 2020

M6 delta Insert1

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox