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Ribonucleotide reductase
From Proteopedia
(Difference between revisions)
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'''Ribonucleotide reductase''' (RNR) or '''ribonucleotide-diphosphate reductase''' catalyzes the formation of deoxyribonucleotides from ribonucleotides<ref>PMID:16756507</ref>. There are 3 classes of RNR.<br /> | '''Ribonucleotide reductase''' (RNR) or '''ribonucleotide-diphosphate reductase''' catalyzes the formation of deoxyribonucleotides from ribonucleotides<ref>PMID:16756507</ref>. There are 3 classes of RNR.<br /> | ||
* '''Class I RNR''' is a tetramer composed from large (RNR1) and small (RNR2) subunits. Class I RNR is iron-dependent and produces tyrosyl radical. Thimidine triphosphate (TTP) is an effector in the reaction.<br /> | * '''Class I RNR''' is a tetramer composed from large (RNR1) and small (RNR2) subunits. Class I RNR is iron-dependent and produces tyrosyl radical. Thimidine triphosphate (TTP) is an effector in the reaction.<br /> | ||
| + | * ''' Class Ib RNR''' contains 2 proteins: α (NrdE) and β (NrdF).<br /> | ||
* ''' Class II RNR''' reduces ribonucleotide triphosphates using coenzyme B12.<br /> | * ''' Class II RNR''' reduces ribonucleotide triphosphates using coenzyme B12.<br /> | ||
* '''Class III RNR''' generate glycine radical using S-adenosyl methionine and Fe-S center.<br /> | * '''Class III RNR''' generate glycine radical using S-adenosyl methionine and Fe-S center.<br /> | ||
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**[[3hne]], [[3hnf]] – hRNR M1 + ATP + TTP<BR /> | **[[3hne]], [[3hnf]] – hRNR M1 + ATP + TTP<BR /> | ||
**[[2wgh]], [[5d1y]] - hRNR M1 + ATP + Mg<BR /> | **[[2wgh]], [[5d1y]] - hRNR M1 + ATP + Mg<BR /> | ||
| - | **[[4x3v]] - hRNR M1 + inhibitor<br /> | + | **[[6aui]] - hRNR M1 + ATP + CDP<br /> |
| + | **[[5tus]], [[4x3v]] - hRNR M1 + inhibitor + TTP<br /> | ||
**[[3paw]] - yRNR R1 + ATP<BR /> | **[[3paw]] - yRNR R1 + ATP<BR /> | ||
**[[3k8t]] - yRNR R1 + ADP analog<BR /> | **[[3k8t]] - yRNR R1 + ADP analog<BR /> | ||
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**[[2ani]] - CtRNR R2 (mutant) + Fe + Pb<BR /> | **[[2ani]] - CtRNR R2 (mutant) + Fe + Pb<BR /> | ||
**[[4m1h]] - CtRNR R2 <br /> | **[[4m1h]] - CtRNR R2 <br /> | ||
| - | **[[4dr0]] - RNR + Mn – Bacillus subtilis<br /> | ||
**[[1uzr]] – RNR R2 – ''Mycobacterium tuberculosis''<BR /> | **[[1uzr]] – RNR R2 – ''Mycobacterium tuberculosis''<BR /> | ||
**[[3olj]] – hRNR M2<br /> | **[[3olj]] – hRNR M2<br /> | ||
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**[[3vpm]], [[3vpn]], [[3vpo]] – hRNR M2 (mutant)<br /> | **[[3vpm]], [[3vpn]], [[3vpo]] – hRNR M2 (mutant)<br /> | ||
**[[2rcc]] – RNR – ''Bacillus halodurans''<br /> | **[[2rcc]] – RNR – ''Bacillus halodurans''<br /> | ||
| - | **[[4bmq]], [[4bmr]], [[4bmt]] - BcRNR + Fe – ''Bacillus cereus''<br /> | ||
| - | **[[4bmu]] - BcRNR + Mn<br /> | ||
**[[4n83]] - SsRNR + Mn<br /> | **[[4n83]] - SsRNR + Mn<br /> | ||
| + | **[[4hr5]] - GkRNR – ''Geobacillus kaustophilus''<br /> | ||
| + | **[[5omj]], [[5omk]] - GkRNR + Fe <br /> | ||
| + | **[[4hr0]], [[4hr4]] - GkRNR + Fe + Mn <br /> | ||
| + | **[[6f6c]], [[6f6e]], [[6f6f]], [[6f6g]], [[6f6h]], [[6f6k]] - GkRNR (mutant) + Fe + Mn <br /> | ||
| + | **[[6cwo]], [[6cwq]] - FjRNR + Mn – ''Flavobacterium johnsoniae''<br /> | ||
*''Ribonucleotide reductase small subunit complex'' | *''Ribonucleotide reductase small subunit complex'' | ||
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**[[5ci3]], [[5ci0]], [[5ci1]], [[5ci2]] – EcRNR R2 (mutant) + fluoro-tyrosine + FE<sub>2</sub>O<br /> | **[[5ci3]], [[5ci0]], [[5ci1]], [[5ci2]] – EcRNR R2 (mutant) + fluoro-tyrosine + FE<sub>2</sub>O<br /> | ||
**[[1w69]] – mRNR R2 + acetate<br /> | **[[1w69]] – mRNR R2 + acetate<br /> | ||
| - | **[[ | + | **[[6cwp]] – FjRNR + Mn + peptide<br /> |
*Ribonucleotide reductase small+large subunit | *Ribonucleotide reductase small+large subunit | ||
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**[[5cns]], [[5cnt]], [[5cnv]], [[5cnu]] - EcRNR R1 + R2 + 2 nucleotides<br /> | **[[5cns]], [[5cnt]], [[5cnv]], [[5cnu]] - EcRNR R1 + R2 + 2 nucleotides<br /> | ||
**[[2bq1]] - StRNR R1+ R2<br /> | **[[2bq1]] - StRNR R1+ R2<br /> | ||
| - | **[[5im3]] - RNR + dATP – ''Pseudomonas aeruginosa''<br /> | + | **[[5im3]] - RNR + dATP – ''Pseudomonas aeruginosa''<br /> |
| + | |||
| + | Class Ib RNR | ||
| + | |||
| + | **[[6cgm]] - BaRNR subunit NrdE – ''Bacillus subtilis''<br /> | ||
| + | **[[6cgn]], [[6cgl]] - BaRNR subunit NrdE + AMP derivative <br /> | ||
| + | **[[4dr0]] - BaRNR subunit NrdF + Mn <br /> | ||
| + | **[[4bmq]], [[4bmr]], [[4bmt]] - BcRNR NrdF + Fe – ''Bacillus cereus''<br /> | ||
| + | **[[4bmu]] - BcRNR NrdF + Mn<br /> | ||
| + | **[[4bmo]], [[4bmp]] - BcRNR NrdF + NrdI + Fe + FMN<br /> | ||
*'''Class II ribonucleotide reductase''' | *'''Class II ribonucleotide reductase''' | ||
Revision as of 08:19, 15 October 2018
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3D Structures of Ribonucleotide reductase
Updated on 15-October-2018
Class Ib RNR
References
- ↑ Nordlund P, Reichard P. Ribonucleotide reductases. Annu Rev Biochem. 2006;75:681-706. PMID:16756507 doi:http://dx.doi.org/10.1146/annurev.biochem.75.103004.142443
- ↑ Munro JB, Silva JC. Ribonucleotide reductase as a target to control apicomplexan diseases. Curr Issues Mol Biol. 2012;14(1):9-26. Epub 2011 Jul 26. PMID:21791713
- ↑ Larsson KM, Jordan A, Eliasson R, Reichard P, Logan DT, Nordlund P. Structural mechanism of allosteric substrate specificity regulation in a ribonucleotide reductase. Nat Struct Mol Biol. 2004 Nov;11(11):1142-9. Epub 2004 Oct 10. PMID:15475969 doi:http://dx.doi.org/10.1038/nsmb838
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