2qvb

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[[Image:2qvb.jpg|left|200px]]
[[Image:2qvb.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2qvb |SIZE=350|CAPTION= <scene name='initialview01'>2qvb</scene>, resolution 1.190&Aring;
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The line below this paragraph, containing "STRUCTURE_2qvb", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:Cl+Binding+Site+For+Residue+A+2'>AC1</scene>, <scene name='pdbsite=AC2:Cl+Binding+Site+For+Residue+B+2'>AC2</scene>, <scene name='pdbsite=AC3:Edo+Binding+Site+For+Residue+A+1'>AC3</scene> and <scene name='pdbsite=AC4:Edo+Binding+Site+For+Residue+B+1'>AC4</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Haloalkane_dehalogenase Haloalkane dehalogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.8.1.5 3.8.1.5] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= dhaA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])
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-->
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|DOMAIN=
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{{STRUCTURE_2qvb| PDB=2qvb | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qvb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qvb OCA], [http://www.ebi.ac.uk/pdbsum/2qvb PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2qvb RCSB]</span>
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}}
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'''Crystal Structure of Haloalkane Dehalogenase Rv2579 from Mycobacterium tuberculosis'''
'''Crystal Structure of Haloalkane Dehalogenase Rv2579 from Mycobacterium tuberculosis'''
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[[Category: Mazumdar, P A.]]
[[Category: Mazumdar, P A.]]
[[Category: TBSGC, TB Structural Genomics Consortium.]]
[[Category: TBSGC, TB Structural Genomics Consortium.]]
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[[Category: alpha-beta hydrolase protein]]
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[[Category: Alpha-beta hydrolase protein]]
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[[Category: haloalkane dehalogenase]]
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[[Category: Haloalkane dehalogenase]]
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[[Category: rv2579]]
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[[Category: Rv2579]]
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[[Category: tb structural genomics consortium]]
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[[Category: Tb structural genomics consortium]]
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[[Category: tbsgc]]
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[[Category: Tbsgc]]
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[[Category: x-ray crystallography]]
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[[Category: X-ray crystallography]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 15:45:01 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:53:43 2008''
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Revision as of 12:45, 4 May 2008

Image:2qvb.jpg

Template:STRUCTURE 2qvb

Crystal Structure of Haloalkane Dehalogenase Rv2579 from Mycobacterium tuberculosis


Overview

Haloalkane dehalogenases are enzymes well known to be important in bioremediation; the organisms from which they are produced are able to clean up toxic organohalides from polluted environments. However, besides being found in such contaminated environments, these enzymes have also been found in root or tissue-colonizing bacterial species. The haloalkane dehalogenase Rv2579 from Mycobacterium tuberculosis H37Rv has been cloned, expressed, purified and its crystal structure determined at high resolution (1.2A). In addition, the crystal structure of the enzyme has been determined in complex with the product from the reaction with 1,3-dibromopropane, i.e. 1,3-propanediol and in complex with the classical substrate of haloalkane dehalogenases, 1,2-dichloroethane. The enzyme is a two-domain protein having a catalytic domain of an alpha/beta hydrolase fold and a cap domain. The active site residues and the halide-stabilizing residues have been identified as Asp109, Glu133, His273, Asn39 and Trp110. Its overall structure is similar to those of other known haloalkane dehalogenases. Its mechanism of action involves an S(N)2 nucleophilic displacement.

About this Structure

2QVB is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.

Reference

X-ray crystal structure of Mycobacterium tuberculosis haloalkane dehalogenase Rv2579., Mazumdar PA, Hulecki JC, Cherney MM, Garen CR, James MN, Biochim Biophys Acta. 2008 Feb;1784(2):351-62. Epub 2007 Nov 13. PMID:18062934 Page seeded by OCA on Sun May 4 15:45:01 2008

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