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2r2i
From Proteopedia
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'''Myristoylated Guanylate Cyclase Activating Protein-1 with Calcium Bound''' | '''Myristoylated Guanylate Cyclase Activating Protein-1 with Calcium Bound''' | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Stephen, R.]] | [[Category: Stephen, R.]] | ||
| - | [[Category: | + | [[Category: Calcium]] |
| - | [[Category: | + | [[Category: Ef hand]] |
| - | [[Category: | + | [[Category: Gcap]] |
| - | [[Category: | + | [[Category: Gcap-1]] |
| - | [[Category: | + | [[Category: Gcap1]] |
| - | [[Category: | + | [[Category: Guanylate cyclase activating protein]] |
| - | [[Category: | + | [[Category: Lipoprotein]] |
| - | [[Category: | + | [[Category: Lyase activator]] |
| - | [[Category: | + | [[Category: Myristate]] |
| - | [[Category: | + | [[Category: Sensory transduction]] |
| - | [[Category: | + | [[Category: Vision]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 16:07:31 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 13:07, 4 May 2008
Myristoylated Guanylate Cyclase Activating Protein-1 with Calcium Bound
Overview
Guanylate cyclase-activating proteins (GCAPs) are Ca(2+)-binding proteins myristoylated at the N terminus that regulate guanylate cyclases in photoreceptor cells and belong to the family of neuronal calcium sensors (NCS). Many NCS proteins display a recoverin-like "calcium-myristoyl switch" whereby the myristoyl group, buried inside the protein in the Ca(2+)-free state, becomes fully exposed upon Ca(2+) binding. Here we present a 2.0 A resolution crystal structure of myristoylated GCAP1 with Ca(2+) bound. The acyl group is buried inside Ca(2+)-bound GCAP1. This is in sharp contrast to Ca(2+)-bound recoverin, where the myristoyl group is solvent exposed. Furthermore, we provide direct evidence that the acyl group in GCAP1 remains buried in the Ca(2+)-free state and does not undergo switching. A pronounced kink in the C-terminal helix and the presence of the myristoyl group allow clustering of sequence elements crucial for GCAP1 activity.
About this Structure
2R2I is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.
Reference
Stabilizing function for myristoyl group revealed by the crystal structure of a neuronal calcium sensor, guanylate cyclase-activating protein 1., Stephen R, Bereta G, Golczak M, Palczewski K, Sousa MC, Structure. 2007 Nov;15(11):1392-402. PMID:17997965 Page seeded by OCA on Sun May 4 16:07:31 2008
