2xvo

From Proteopedia

(Difference between revisions)

Revision as of 12:22, 22 July 2020

SSO1725, a protein involved in the CRISPR/Cas pathway

Structural highlights

2xvo is a 4 chain structure with sequence from 'saccharolobus solfataricus'. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CMR7B_SULSO] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (By similarity).

Publication Abstract from PubMed

The prokaryotic clusters of regularly interspaced palindromic repeats (CRISPR) system utilizes genomically encoded CRISPR RNA (crRNA), derived from invading viruses and incorporated into ribonucleoprotein complexes with CRISPR-associated (CAS) proteins, to target and degrade viral DNA or RNA on subsequent infection. RNA is targeted by the CMR complex. In Sulfolobus solfataricus, this complex is composed of seven CAS protein subunits (Cmr1-7) and carries a diverse "payload" of targeting crRNA. The crystal structure of Cmr7 and low-resolution structure of the complex are presented. S. solfataricus CMR cleaves RNA targets in an endonucleolytic reaction at UA dinucleotides. This activity is dependent on the 8 nt repeat-derived 5' sequence in the crRNA, but not on the presence of a protospacer-associated motif (PAM) in the target. Both target and guide RNAs can be cleaved, although a single molecule of guide RNA can support the degradation of multiple targets.

Structure and mechanism of the CMR complex for CRISPR-mediated antiviral immunity.,Zhang J, Rouillon C, Kerou M, Reeks J, Brugger K, Graham S, Reimann J, Cannone G, Liu H, Albers SV, Naismith JH, Spagnolo L, White MF Mol Cell. 2012 Feb 10;45(3):303-13. doi: 10.1016/j.molcel.2011.12.013. Epub 2012 , Jan 5. PMID:22227115^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zhang J, Rouillon C, Kerou M, Reeks J, Brugger K, Graham S, Reimann J, Cannone G, Liu H, Albers SV, Naismith JH, Spagnolo L, White MF. Structure and mechanism of the CMR complex for CRISPR-mediated antiviral immunity. Mol Cell. 2012 Feb 10;45(3):303-13. doi: 10.1016/j.molcel.2011.12.013. Epub 2012 , Jan 5. PMID:22227115 doi:http://dx.doi.org/10.1016/j.molcel.2011.12.013

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2xvo"

@@ Line 1: / Line 1: @@
 ==SSO1725, a protein involved in the CRISPR/Cas pathway==
-<StructureSection load='2xvo' size='340' side='right' caption='[[2xvo]], [[Resolution|resolution]] 2.08&Aring;' scene=''>
+<StructureSection load='2xvo' size='340' side='right'caption='[[2xvo]], [[Resolution|resolution]] 2.08&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2xvo]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/'saccharolobus_solfataricus' 'saccharolobus solfataricus']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XVO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XVO FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2xvo]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/'saccharolobus_solfataricus' 'saccharolobus solfataricus']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XVO OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2XVO FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xvo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xvo OCA], [http://pdbe.org/2xvo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xvo RCSB], [http://www.ebi.ac.uk/pdbsum/2xvo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xvo ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2xvo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xvo OCA], [http://pdbe.org/2xvo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xvo RCSB], [http://www.ebi.ac.uk/pdbsum/2xvo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xvo ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 23: / Line 23: @@
 </StructureSection>
 [[Category: Saccharolobus solfataricus]]
+[[Category: Large Structures]]
 [[Category: Liu, H]]
 [[Category: McMahon, S]]

2xvo

From Proteopedia

Revision as of 12:22, 22 July 2020

SSO1725, a protein involved in the CRISPR/Cas pathway

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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