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2xog

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Revision as of 12:14, 22 July 2020

Functional and Structural Analyses of N-Acylsulfonamide-Linked Dinucleoside Inhibitors of Ribonuclease A

Structural highlights

2xog is a 2 chain structure with sequence from Bos taurus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	1ssb, 1afu, 1w4q, 1c0b, 6rat, 1ymr, 1o0o, 8rsa, 5rsa, 2w5l, 1rno, 7rat, 1izq, 1eid, 1cjq, 1eos, 1rnn, 1j82, 1qhc, 1ymw, 1o0h, 1afk, 1f0v, 1dy5, 1rnq, 1jn4, 1rnm, 1rbf, 1z3m, 1rnx, 1rob, 1rbn, 1rbx, 1eow, 1rbi, 7rsa, 1rbj, 1dfj, 1rsm, 1kf5, 1xps, 1rat, 1fev, 2aas, 1rhb, 1j80, 3srn, 1b6v, 1fs3, 1rpg, 1z6s, 1kf8, 1rtb, 4rsk, 1bzq, 9rsa, 1bel, 1kf4, 1kf7, 1rbw, 9rat, 1eie, 1rpf, 1o0m, 1c8w, 1rnc, 1rca, 1c0c, 1jvt, 3rsd, 1xpt, 1rbb, 2blz, 3rsp, 1ssc, 8rat, 2apq, 1j7z, 1w4o, 1rbd, 1rnz, 1jvv, 1rar, 1kf3, 1z3l, 1aqp, 1rbh, 2w5m, 1rbg, 1o0n, 1js0, 3rsk, 1ssa, 1cjr, 1eic, 1d5d, 1ymn, 1j81, 1rnw, 2w5k, 1rph, 2rns, 1rta, 1rnu, 1rny, 1z3p, 1gv7, 1rcn, 1rnv, 4rat, 1w4p, 4rsd, 1a5p, 1wbu, 1rha, 1ras, 2blp, 1rnd, 1kf2, 1d5h, 1srn, 1d5e, 2rat, 1izp, 2w5g, 1o0f, 1izr, 1c9x, 2rln, 4srn, 1c9v, 1u1b, 5rat, 1ruv, 1rbc, 2w5i, 1afl, 1z6d, 1rbe, 1a5q, 1kh8, 1lsq, 1jvu, 1a2w, 3rn3, 3rat
Activity:	Pancreatic ribonuclease, with EC number 3.1.27.5
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RNAS1_BOVIN] Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA.^[1]

Publication Abstract from PubMed

Molecular probes are useful for both studying and controlling the functions of enzymes and other proteins. The most useful probes have high affinity for their target, along with small size and resistance to degradation. Here, we report on new surrogates for nucleic acids that fulfill these criteria. Isosteres in which phosphoryl [R-O-P(O(2) (-) )-O-R'] groups are replaced with N-acylsulfonamidyl [R-C(O)-N(-) -S(O(2) )-R'] or sulfonimidyl [R-S(O(2) )-N(-) -S(O(2) )-R'] groups increase the number of nonbridging oxygens from two (phosphoryl) to three (N-acylsulfonamidyl) or four (sulfonimidyl). Six such isosteres were found to be more potent inhibitors of catalysis by bovine pancreatic RNase A than are parent compounds containing phosphoryl groups. The atomic structures of two RNase A.N-acylsulfonamide complexes were determined at high resolution by X-ray crystallography. The N-acylsulfonamidyl groups were observed to form more hydrogen bonds with active site residues than did the phosphoryl groups in analogous complexes. These data encourage the further development and use of N-acylsulfonamides and sulfonimides as antagonists of nucleic acid-binding proteins.

Functional and structural analyses of N-acylsulfonamide-linked dinucleoside inhibitors of RNase A.,Thiyagarajan N, Smith BD, Raines RT, Acharya KR FEBS J. 2010 Dec 4. doi: 10.1111/j.1742-4658.2010.07976.x. PMID:21205197^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ delCardayre SB, Ribo M, Yokel EM, Quirk DJ, Rutter WJ, Raines RT. Engineering ribonuclease A: production, purification and characterization of wild-type enzyme and mutants at Gln11. Protein Eng. 1995 Mar;8(3):261-73. PMID:7479688
↑ Thiyagarajan N, Smith BD, Raines RT, Acharya KR. Functional and structural analyses of N-acylsulfonamide-linked dinucleoside inhibitors of RNase A. FEBS J. 2010 Dec 4. doi: 10.1111/j.1742-4658.2010.07976.x. PMID:21205197 doi:10.1111/j.1742-4658.2010.07976.x

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2xog"

@@ Line 1: / Line 1: @@
 ==Functional and Structural Analyses of N-Acylsulfonamide-Linked Dinucleoside Inhibitors of Ribonuclease A==
-<StructureSection load='2xog' size='340' side='right' caption='[[2xog]], [[Resolution|resolution]] 1.72&Aring;' scene=''>
+<StructureSection load='2xog' size='340' side='right'caption='[[2xog]], [[Resolution|resolution]] 1.72&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2xog]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XOG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XOG FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2xog]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XOG OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2XOG FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SFB:(2S,3S,4R,5R)-5-(6-AMINOPURIN-9-YL)-N-[[(2S,3S,4R,5R)-5-(2,4-DIOXOPYRIMIDIN-1-YL)-4-HYDROXY-2-(HYDROXYMETHYL)OXOLAN-3-YL]METHYLSULFONYL]-3,4-DIHYDROXY-OXOLANE-2-CARBOXAMIDE'>SFB</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SFB:(2S,3S,4R,5R)-5-(6-AMINOPURIN-9-YL)-N-[[(2S,3S,4R,5R)-5-(2,4-DIOXOPYRIMIDIN-1-YL)-4-HYDROXY-2-(HYDROXYMETHYL)OXOLAN-3-YL]METHYLSULFONYL]-3,4-DIHYDROXY-OXOLANE-2-CARBOXAMIDE'>SFB</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ssb|1ssb]], [[1afu|1afu]], [[1w4q|1w4q]], [[1c0b|1c0b]], [[6rat|6rat]], [[1ymr|1ymr]], [[1o0o|1o0o]], [[8rsa|8rsa]], [[5rsa|5rsa]], [[2w5l|2w5l]], [[1rno|1rno]], [[7rat|7rat]], [[1izq|1izq]], [[1eid|1eid]], [[1cjq|1cjq]], [[1eos|1eos]], [[1rnn|1rnn]], [[1j82|1j82]], [[1qhc|1qhc]], [[1ymw|1ymw]], [[1o0h|1o0h]], [[1afk|1afk]], [[1f0v|1f0v]], [[1dy5|1dy5]], [[1rnq|1rnq]], [[1jn4|1jn4]], [[1rnm|1rnm]], [[1rbf|1rbf]], [[1z3m|1z3m]], [[1rnx|1rnx]], [[1rob|1rob]], [[1rbn|1rbn]], [[1rbx|1rbx]], [[1eow|1eow]], [[1rbi|1rbi]], [[7rsa|7rsa]], [[1rbj|1rbj]], [[1dfj|1dfj]], [[1rsm|1rsm]], [[1kf5|1kf5]], [[1xps|1xps]], [[1rat|1rat]], [[1fev|1fev]], [[2aas|2aas]], [[1rhb|1rhb]], [[1j80|1j80]], [[3srn|3srn]], [[1b6v|1b6v]], [[1fs3|1fs3]], [[1rpg|1rpg]], [[1z6s|1z6s]], [[1kf8|1kf8]], [[1rtb|1rtb]], [[4rsk|4rsk]], [[1bzq|1bzq]], [[9rsa|9rsa]], [[1bel|1bel]], [[1kf4|1kf4]], [[1kf7|1kf7]], [[1rbw|1rbw]], [[9rat|9rat]], [[1eie|1eie]], [[1rpf|1rpf]], [[1o0m|1o0m]], [[1c8w|1c8w]], [[1rnc|1rnc]], [[1rca|1rca]], [[1c0c|1c0c]], [[1jvt|1jvt]], [[3rsd|3rsd]], [[1xpt|1xpt]], [[1rbb|1rbb]], [[2blz|2blz]], [[3rsp|3rsp]], [[1ssc|1ssc]], [[8rat|8rat]], [[2apq|2apq]], [[1j7z|1j7z]], [[1w4o|1w4o]], [[1rbd|1rbd]], [[1rnz|1rnz]], [[1jvv|1jvv]], [[1rar|1rar]], [[1kf3|1kf3]], [[1z3l|1z3l]], [[1aqp|1aqp]], [[1rbh|1rbh]], [[2w5m|2w5m]], [[1rbg|1rbg]], [[1o0n|1o0n]], [[1js0|1js0]], [[3rsk|3rsk]], [[1ssa|1ssa]], [[1cjr|1cjr]], [[1eic|1eic]], [[1d5d|1d5d]], [[1ymn|1ymn]], [[1j81|1j81]], [[1rnw|1rnw]], [[2w5k|2w5k]], [[1rph|1rph]], [[2rns|2rns]], [[1rta|1rta]], [[1rnu|1rnu]], [[1rny|1rny]], [[1z3p|1z3p]], [[1gv7|1gv7]], [[1rcn|1rcn]], [[1rnv|1rnv]], [[4rat|4rat]], [[1w4p|1w4p]], [[4rsd|4rsd]], [[1a5p|1a5p]], [[1wbu|1wbu]], [[1rha|1rha]], [[1ras|1ras]], [[2blp|2blp]], [[1rnd|1rnd]], [[1kf2|1kf2]], [[1d5h|1d5h]], [[1srn|1srn]], [[1d5e|1d5e]], [[2rat|2rat]], [[1izp|1izp]], [[2w5g|2w5g]], [[1o0f|1o0f]], [[1izr|1izr]], [[1c9x|1c9x]], [[2rln|2rln]], [[4srn|4srn]], [[1c9v|1c9v]], [[1u1b|1u1b]], [[5rat|5rat]], [[1ruv|1ruv]], [[1rbc|1rbc]], [[2w5i|2w5i]], [[1afl|1afl]], [[1z6d|1z6d]], [[1rbe|1rbe]], [[1a5q|1a5q]], [[1kh8|1kh8]], [[1lsq|1lsq]], [[1jvu|1jvu]], [[1a2w|1a2w]], [[3rn3|3rn3]], [[3rat|3rat]]</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xog FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xog OCA], [http://pdbe.org/2xog PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xog RCSB], [http://www.ebi.ac.uk/pdbsum/2xog PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xog ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2xog FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xog OCA], [http://pdbe.org/2xog PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xog RCSB], [http://www.ebi.ac.uk/pdbsum/2xog PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xog ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 22: / Line 22: @@
 ==See Also==
-*[[Ribonuclease|Ribonuclease]]
+*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
 *[[Temp|Temp]]
 == References ==
@@ Line 29: / Line 29: @@
 </StructureSection>
 [[Category: Bos taurus]]
+[[Category: Large Structures]]
 [[Category: Pancreatic ribonuclease]]
 [[Category: Acharya, K R]]

2xog

From Proteopedia

Revision as of 12:14, 22 July 2020

Functional and Structural Analyses of N-Acylsulfonamide-Linked Dinucleoside Inhibitors of Ribonuclease A

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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