2r4v

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[[Image:2r4v.jpg|left|200px]]
[[Image:2r4v.jpg|left|200px]]
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{{Structure
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|PDB= 2r4v |SIZE=350|CAPTION= <scene name='initialview01'>2r4v</scene>, resolution 1.85&Aring;
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The line below this paragraph, containing "STRUCTURE_2r4v", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=GTT:GLUTATHIONE'>GTT</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= CLIC2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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|DOMAIN=
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{{STRUCTURE_2r4v| PDB=2r4v | SCENE= }}
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|RELATEDENTRY=[[2r5g|2R5G]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2r4v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r4v OCA], [http://www.ebi.ac.uk/pdbsum/2r4v PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2r4v RCSB]</span>
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'''Structure of human CLIC2, crystal form A'''
'''Structure of human CLIC2, crystal form A'''
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[[Category: Hansen, G.]]
[[Category: Hansen, G.]]
[[Category: Parker, M W.]]
[[Category: Parker, M W.]]
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[[Category: chloride channel]]
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[[Category: Chloride channel]]
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[[Category: chloride intracellular channel]]
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[[Category: Chloride intracellular channel]]
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[[Category: clic2]]
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[[Category: Clic2]]
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[[Category: ion transport]]
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[[Category: Ion transport]]
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[[Category: ionic channel]]
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[[Category: Ionic channel]]
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[[Category: pore-forming protein]]
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[[Category: Pore-forming protein]]
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[[Category: ryanodine receptor]]
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[[Category: Ryanodine receptor]]
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[[Category: transport]]
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[[Category: Transport]]
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[[Category: transport protein]]
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[[Category: Transport protein]]
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[[Category: voltage-gated channel]]
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[[Category: Voltage-gated channel]]
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[[Category: x-ray crystallography]]
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[[Category: X-ray crystallography]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 16:14:20 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:56:33 2008''
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Revision as of 13:14, 4 May 2008

Image:2r4v.jpg

Template:STRUCTURE 2r4v

Structure of human CLIC2, crystal form A


Overview

Chloride intracellular channel (CLIC) proteins possess the remarkable property of being able to convert from a water-soluble state to a membrane channel state. We determined the three-dimensional structure of human CLIC2 in its water-soluble form by X-ray crystallography at 1.8-A resolution from two crystal forms. In contrast to the previously characterized CLIC1 protein, which forms a possibly functionally important disulfide-induced dimer under oxidizing conditions, we show that CLIC2 possesses an intramolecular disulfide and that the protein remains monomeric irrespective of redox conditions. Site-directed mutagenesis studies show that removal of the intramolecular disulfide or introduction of cysteine residues in CLIC2, equivalent to those that form the intramolecular disulfide in CLIC1, does not cause dimer formation under oxidizing conditions. We also show that CLIC2 forms pH-dependent chloride channels in vitro with higher channel activity at low pH levels and that the channels are subject to redox regulation. In both crystal forms, we observed an extended loop region from the C-terminal domain, called the foot loop, inserting itself into an interdomain crevice of a neighboring molecule. The equivalent region in the structurally related glutathione transferase superfamily corresponds to the active site. This so-called foot-in-mouth interaction suggests that CLIC2 might recognize other proteins such as the ryanodine receptor through a similar interaction.

About this Structure

2R4V is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structure of the Janus protein human CLIC2., Cromer BA, Gorman MA, Hansen G, Adams JJ, Coggan M, Littler DR, Brown LJ, Mazzanti M, Breit SN, Curmi PM, Dulhunty AF, Board PG, Parker MW, J Mol Biol. 2007 Nov 30;374(3):719-31. Epub 2007 Sep 20. PMID:17945253 Page seeded by OCA on Sun May 4 16:14:20 2008

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