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2yc2

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Revision as of 12:43, 22 July 2020

Intraflagellar Transport Complex 25-27 from Chlamydomonas

Structural highlights

2yc2 is a 4 chain structure with sequence from Chlre. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	2yc4
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[IFT27_CHLRE] Small GTPase-like component of the intraflagellar transport (IFT) complex B. Forms a subcomplex within the IFT complex B with IFT25. Has very low GTPase activity either because it lacks the conserved catalytic Gln in position 79 or because it requires some GTPase-activating protein (GAP) for GTP turnover.^[1]

Publication Abstract from PubMed

The cilium is an important organelle that is found on many eukaryotic cells, where it serves essential functions in motility, sensory reception and signalling. Intraflagellar transport (IFT) is a vital process for the formation and maintenance of cilia. We have determined the crystal structure of Chlamydomonas reinhardtii IFT25/27, an IFT sub-complex, at 2.6 A resolution. IFT25 and IFT27 interact via a conserved interface that we verify biochemically using structure-guided mutagenesis. IFT27 displays the fold of Rab-like small guanosine triphosphate hydrolases (GTPases), binds GTP and GDP with micromolar affinity and has very low intrinsic GTPase activity, suggesting that it likely requires a GTPase-activating protein (GAP) for robust GTP turnover. A patch of conserved surface residues contributed by both IFT25 and IFT27 is found adjacent to the GTP-binding site and could mediate the binding to other IFT proteins as well as to a potential GAP. These results provide the first step towards a high-resolution structural understanding of the IFT complex.

Crystal structure of the intraflagellar transport complex 25/27.,Bhogaraju S, Taschner M, Morawetz M, Basquin C, Lorentzen E EMBO J. 2011 Apr 19. PMID:21505417^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Bhogaraju S, Taschner M, Morawetz M, Basquin C, Lorentzen E. Crystal structure of the intraflagellar transport complex 25/27. EMBO J. 2011 Apr 19. PMID:21505417 doi:10.1038/emboj.2011.110
↑ Bhogaraju S, Taschner M, Morawetz M, Basquin C, Lorentzen E. Crystal structure of the intraflagellar transport complex 25/27. EMBO J. 2011 Apr 19. PMID:21505417 doi:10.1038/emboj.2011.110

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2yc2"

@@ Line 1: / Line 1: @@
 ==Intraflagellar Transport Complex 25-27 from Chlamydomonas==
-<StructureSection load='2yc2' size='340' side='right' caption='[[2yc2]], [[Resolution|resolution]] 2.59&Aring;' scene=''>
+<StructureSection load='2yc2' size='340' side='right'caption='[[2yc2]], [[Resolution|resolution]] 2.59&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2yc2]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlre Chlre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YC2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YC2 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2yc2]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlre Chlre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YC2 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2YC2 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2yc4|2yc4]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2yc2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yc2 OCA], [http://pdbe.org/2yc2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2yc2 RCSB], [http://www.ebi.ac.uk/pdbsum/2yc2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2yc2 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2yc2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yc2 OCA], [http://pdbe.org/2yc2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2yc2 RCSB], [http://www.ebi.ac.uk/pdbsum/2yc2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2yc2 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 24: / Line 24: @@
 </StructureSection>
 [[Category: Chlre]]
+[[Category: Large Structures]]
 [[Category: Bhogaraju, S]]
 [[Category: Lorentzen, E]]

2yc2

From Proteopedia

Revision as of 12:43, 22 July 2020

Intraflagellar Transport Complex 25-27 from Chlamydomonas

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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