2r9a

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[[Image:2r9a.jpg|left|200px]]
[[Image:2r9a.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2r9a |SIZE=350|CAPTION= <scene name='initialview01'>2r9a</scene>, resolution 2.50&Aring;
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The line below this paragraph, containing "STRUCTURE_2r9a", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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|GENE= NHEJ1, XLF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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|DOMAIN=
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{{STRUCTURE_2r9a| PDB=2r9a | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2r9a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r9a OCA], [http://www.ebi.ac.uk/pdbsum/2r9a PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2r9a RCSB]</span>
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'''Crystal structure of human XLF'''
'''Crystal structure of human XLF'''
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[[Category: Andres, S N.]]
[[Category: Andres, S N.]]
[[Category: Junop, M S.]]
[[Category: Junop, M S.]]
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[[Category: alternative splicing]]
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[[Category: Alternative splicing]]
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[[Category: cernunnos,non-homologous end joining]]
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[[Category: Cernunnos,non-homologous end joining]]
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[[Category: disease mutation]]
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[[Category: Disease mutation]]
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[[Category: dna damage]]
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[[Category: Dna damage]]
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[[Category: dna double strand break repair]]
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[[Category: Dna double strand break repair]]
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[[Category: dna repair]]
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[[Category: Dna repair]]
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[[Category: nucleus]]
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[[Category: Nucleus]]
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[[Category: protein binding]]
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[[Category: Protein binding]]
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[[Category: xlf]]
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[[Category: Xlf]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 16:27:24 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:57:46 2008''
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Revision as of 13:27, 4 May 2008

Image:2r9a.jpg

Template:STRUCTURE 2r9a

Crystal structure of human XLF


Overview

DNA double-strand breaks represent one of the most severe forms of DNA damage in mammalian cells. One pathway for repairing these breaks occurs via nonhomologous end-joining (NHEJ) and depends on XRCC4, LigaseIV, and Cernunnos, also called XLF. Although XLF stimulates XRCC4/LigaseIV to ligate mismatched and noncohesive DNA ends, the mechanistic basis for this function remains unclear. Here we report the structure of a partially functional 224 residue N-terminal fragment of human XLF. Despite only weak sequence similarity, XLF(1-170) shares structural homology with XRCC4(1-159). However, unlike the highly extended 130 A helical domain observed in XRCC4, XLF adopts a more compact, folded helical C-terminal region involving two turns and a twist, wrapping back to the structurally conserved N terminus. Mutational analysis of XLF and XRCC4 reveals a potential interaction interface, suggesting a mechanism for how XLF stimulates the ligation of mismatched ends.

About this Structure

2R9A is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of human XLF: a twist in nonhomologous DNA end-joining., Andres SN, Modesti M, Tsai CJ, Chu G, Junop MS, Mol Cell. 2007 Dec 28;28(6):1093-101. PMID:18158905 Page seeded by OCA on Sun May 4 16:27:24 2008

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OCA

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