2r9l

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:2r9l.jpg|left|200px]]
[[Image:2r9l.jpg|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 2r9l |SIZE=350|CAPTION= <scene name='initialview01'>2r9l</scene>, resolution 2.400&Aring;
+
The line below this paragraph, containing "STRUCTURE_2r9l", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY=
+
or leave the SCENE parameter empty for the default display.
-
|GENE= Rv0938 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])
+
-->
-
|DOMAIN=
+
{{STRUCTURE_2r9l| PDB=2r9l | SCENE= }}
-
|RELATEDENTRY=[[2iru|2IRU]], [[2irx|2IRX]], [[2iry|2IRY]]
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2r9l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r9l OCA], [http://www.ebi.ac.uk/pdbsum/2r9l PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2r9l RCSB]</span>
+
-
}}
+
'''Polymerase Domain from Mycobacterium tuberculosis Ligase D in complex with DNA'''
'''Polymerase Domain from Mycobacterium tuberculosis Ligase D in complex with DNA'''
Line 29: Line 26:
[[Category: Fox, G C.]]
[[Category: Fox, G C.]]
[[Category: Pitcher, R S.]]
[[Category: Pitcher, R S.]]
-
[[Category: atp-binding]]
+
[[Category: Atp-binding]]
-
[[Category: ligase]]
+
[[Category: Ligase]]
-
[[Category: nucleotide-binding]]
+
[[Category: Nucleotide-binding]]
-
[[Category: protein-dna complex]]
+
[[Category: Protein-dna complex]]
-
[[Category: transferase]]
+
[[Category: Transferase]]
-
[[Category: transferase/dna complex]]
+
[[Category: Transferase/dna complex]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 16:28:34 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:57:54 2008''
+

Revision as of 13:28, 4 May 2008

Image:2r9l.jpg

Template:STRUCTURE 2r9l

Polymerase Domain from Mycobacterium tuberculosis Ligase D in complex with DNA


Overview

Nonhomologous end joining (NHEJ) is a critical DNA double-strand break (DSB) repair pathway required to maintain genome stability. Many prokaryotes possess a minimalist NHEJ apparatus required to repair DSBs during stationary phase, composed of two conserved core proteins, Ku and ligase D (LigD). The crystal structure of Mycobacterium tuberculosis polymerase domain of LigD mediating the synapsis of two noncomplementary DNA ends revealed a variety of interactions, including microhomology base pairing, mismatched and flipped-out bases, and 3' termini forming hairpin-like ends. Biochemical and biophysical studies confirmed that polymerase-induced end synapsis also occurs in solution. We propose that this DNA synaptic structure reflects an intermediate bridging stage of the NHEJ process, before end processing and ligation, with both the polymerase and the DNA sequence playing pivotal roles in determining the sequential order of synapsis and remodeling before end joining.

About this Structure

2R9L is a Protein complex structure of sequences from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.

Reference

Structure of a NHEJ polymerase-mediated DNA synaptic complex., Brissett NC, Pitcher RS, Juarez R, Picher AJ, Green AJ, Dafforn TR, Fox GC, Blanco L, Doherty AJ, Science. 2007 Oct 19;318(5849):456-9. PMID:17947582 Page seeded by OCA on Sun May 4 16:28:34 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2r9l"
Personal tools