This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

6g6a

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Current revision

Crystal structure of a parallel six-helix coiled coil CC-Type2-LL

Structural highlights

6g6a is a 3 chain structure with sequence from Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.701Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

In coiled-coil (CC) protein structures alpha-helices wrap around one another to form rope-like assemblies. Most natural and designed CCs have two-four helices and cyclic (Cn) or dihedral (Dn) symmetry. Increasingly, CCs with five or more helices are being reported. A subset of these higher-order CCs is of interest as they have accessible central channels that can be functionalised; they are alpha-helical barrels. These extended cavities are surprising given the drive to maximise buried hydrophobic surfaces during protein folding and assembly in water. Here, we show that alpha-helical barrels can be maintained by the strategic placement of beta-branched aliphatic residues lining the lumen. Otherwise, the structures collapse or adjust to give more-complex multi-helix assemblies without Cn or Dn symmetry. Nonetheless, the structural hallmark of CCs-namely, knobs-into-holes packing of side chains between helices-is maintained leading to classes of CCs hitherto unobserved in nature or accessed by design.

Maintaining and breaking symmetry in homomeric coiled-coil assemblies.,Rhys GG, Wood CW, Lang EJM, Mulholland AJ, Brady RL, Thomson AR, Woolfson DN Nat Commun. 2018 Oct 8;9(1):4132. doi: 10.1038/s41467-018-06391-y. PMID:30297707^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Rhys GG, Wood CW, Lang EJM, Mulholland AJ, Brady RL, Thomson AR, Woolfson DN. Maintaining and breaking symmetry in homomeric coiled-coil assemblies. Nat Commun. 2018 Oct 8;9(1):4132. doi: 10.1038/s41467-018-06391-y. PMID:30297707 doi:http://dx.doi.org/10.1038/s41467-018-06391-y

1 Structural highlights
2 Publication Abstract from PubMed
3 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6g6a"

Categories: Large Structures | Synthetic construct | Brady RL | Rhys GG | Woolfson DN

@@ Line 1: / Line 1: @@
 ==Crystal structure of a parallel six-helix coiled coil CC-Type2-LL==
-<StructureSection load='6g6a' size='340' side='right' caption='[[6g6a]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
+<StructureSection load='6g6a' size='340' side='right'caption='[[6g6a]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6g6a]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6G6A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6G6A FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6g6a]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6G6A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6G6A FirstGlance]. <br>
-</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.701&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6g6a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6g6a OCA], [http://pdbe.org/6g6a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6g6a RCSB], [http://www.ebi.ac.uk/pdbsum/6g6a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6g6a ProSAT]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6g6a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6g6a OCA], [https://pdbe.org/6g6a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6g6a RCSB], [https://www.ebi.ac.uk/pdbsum/6g6a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6g6a ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">
@@ Line 20: / Line 21: @@
 __TOC__
 </StructureSection>
-[[Category: Brady, R L]]
+[[Category: Large Structures]]
-[[Category: Rhys, G G]]
-[[Category: Woolfson, D N]]
-[[Category: Alpha-helical bundle]]
-[[Category: Coiled coil]]
-[[Category: De novo]]
-[[Category: De novo protein]]
 [[Category: Synthetic construct]]
+[[Category: Brady RL]]
+[[Category: Rhys GG]]
+[[Category: Woolfson DN]]

6g6a

From Proteopedia

Current revision

Crystal structure of a parallel six-helix coiled coil CC-Type2-LL

Structural highlights

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox