2rc6

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[[Image:2rc6.jpg|left|200px]]
[[Image:2rc6.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2rc6 |SIZE=350|CAPTION= <scene name='initialview01'>2rc6</scene>, resolution 2.700&Aring;
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The line below this paragraph, containing "STRUCTURE_2rc6", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:Zn+Binding+Site+For+Residue+A+315'>AC1</scene>, <scene name='pdbsite=AC2:So4+Binding+Site+For+Residue+A+316'>AC2</scene>, <scene name='pdbsite=AC3:So4+Binding+Site+For+Residue+B+316'>AC3</scene>, <scene name='pdbsite=AC4:So4+Binding+Site+For+Residue+C+315'>AC4</scene>, <scene name='pdbsite=AC5:So4+Binding+Site+For+Residue+C+316'>AC5</scene>, <scene name='pdbsite=AC6:So4+Binding+Site+For+Residue+D+315'>AC6</scene>, <scene name='pdbsite=AC7:So4+Binding+Site+For+Residue+D+316'>AC7</scene>, <scene name='pdbsite=AC8:Fad+Binding+Site+For+Residue+A+415'>AC8</scene>, <scene name='pdbsite=AC9:Nap+Binding+Site+For+Residue+A+416'>AC9</scene>, <scene name='pdbsite=BC1:Fad+Binding+Site+For+Residue+B+415'>BC1</scene>, <scene name='pdbsite=BC2:Nap+Binding+Site+For+Residue+B+416'>BC2</scene>, <scene name='pdbsite=BC3:Fad+Binding+Site+For+Residue+C+415'>BC3</scene>, <scene name='pdbsite=BC4:Nap+Binding+Site+For+Residue+C+416'>BC4</scene>, <scene name='pdbsite=BC5:Fad+Binding+Site+For+Residue+D+415'>BC5</scene> and <scene name='pdbsite=BC6:Nap+Binding+Site+For+Residue+D+416'>BC6</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ferredoxin--NADP(+)_reductase Ferredoxin--NADP(+) reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.18.1.2 1.18.1.2] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam00175 NAD_binding_1], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=COG0543 UbiB]</span>
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{{STRUCTURE_2rc6| PDB=2rc6 | SCENE= }}
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|RELATEDENTRY=[[2rc5|2RC5]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rc6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rc6 OCA], [http://www.ebi.ac.uk/pdbsum/2rc6 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2rc6 RCSB]</span>
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}}
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'''Refined structure of FNR from Leptospira interrogans bound to NADP+'''
'''Refined structure of FNR from Leptospira interrogans bound to NADP+'''
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==Reference==
==Reference==
Crystal structures of Leptospira interrogans FAD-containing ferredoxin-NADP+ reductase and its complex with NADP+., Nascimento AS, Catalano-Dupuy DL, Bernardes A, Neto Mde O, Santos MA, Ceccarelli EA, Polikarpov I, BMC Struct Biol. 2007 Oct 24;7:69. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17958910 17958910]
Crystal structures of Leptospira interrogans FAD-containing ferredoxin-NADP+ reductase and its complex with NADP+., Nascimento AS, Catalano-Dupuy DL, Bernardes A, Neto Mde O, Santos MA, Ceccarelli EA, Polikarpov I, BMC Struct Biol. 2007 Oct 24;7:69. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17958910 17958910]
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[[Category: Ferredoxin--NADP(+) reductase]]
 
[[Category: Leptospira interrogans]]
[[Category: Leptospira interrogans]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Nascimento, A S.]]
[[Category: Nascimento, A S.]]
[[Category: Polikarpov, I.]]
[[Category: Polikarpov, I.]]
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[[Category: fnr]]
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[[Category: Fnr]]
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[[Category: oxidoreductase]]
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[[Category: Oxidoreductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 16:38:04 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:58:47 2008''
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Revision as of 13:38, 4 May 2008

Image:2rc6.jpg

Template:STRUCTURE 2rc6

Refined structure of FNR from Leptospira interrogans bound to NADP+


Overview

BACKGROUND: Ferredoxin-NADP(H) reductases (FNRs) are flavoenzymes that catalyze the electron transfer between NADP(H) and the proteins ferredoxin or flavodoxin. A number of structural features distinguish plant and bacterial FNRs, one of which is the mode of the cofactor FAD binding. Leptospira interrogans is a spirochaete parasitic bacterium capable of infecting humans and mammals in general. Leptospira interrogans FNR (LepFNR) displays low sequence identity with plant (34% with Zea mays) and bacterial (31% with Escherichia coli) FNRs. However, LepFNR contains all consensus sequences that define the plastidic class FNRs. RESULTS: The crystal structures of the FAD-containing LepFNR and the complex of the enzyme with NADP+, were solved and compared to known FNRs. The comparison reveals significant structural similarities of the enzyme with the plastidic type FNRs and differences with the bacterial enzymes. Our small angle X-ray scattering experiments show that LepFNR is a monomeric enzyme. Moreover, our biochemical data demonstrate that the LepFNR has an enzymatic activity similar to those reported for the plastidic enzymes and that is significantly different from bacterial flavoenzymes, which display lower turnover rates. CONCLUSION: LepFNR is the first plastidic type FNR found in bacteria and, despite of its low sequence similarity with plastidic FNRs still displays high catalytic turnover rates. The typical structural and biochemical characteristics of plant FNRs unveiled for LepFNR support a notion of a putative lateral gene transfer which presumably offers Leptospira interrogans evolutionary advantages. The wealth of structural information about LepFNR provides a molecular basis for advanced drugs developments against leptospirosis.

About this Structure

2RC6 is a Single protein structure of sequence from Leptospira interrogans. Full crystallographic information is available from OCA.

Reference

Crystal structures of Leptospira interrogans FAD-containing ferredoxin-NADP+ reductase and its complex with NADP+., Nascimento AS, Catalano-Dupuy DL, Bernardes A, Neto Mde O, Santos MA, Ceccarelli EA, Polikarpov I, BMC Struct Biol. 2007 Oct 24;7:69. PMID:17958910 Page seeded by OCA on Sun May 4 16:38:04 2008

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