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| | ==Crystal structure of LokiProfilin1/Rabbit Actin Complex== | | ==Crystal structure of LokiProfilin1/Rabbit Actin Complex== |
| - | <StructureSection load='5yee' size='340' side='right' caption='[[5yee]], [[Resolution|resolution]] 1.81Å' scene=''> | + | <StructureSection load='5yee' size='340' side='right'caption='[[5yee]], [[Resolution|resolution]] 1.81Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5yee]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lokiarchaeum_sp._gc14_75 Lokiarchaeum sp. gc14_75] and [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YEE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YEE FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5yee]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lokiarchaeum_sp._GC14_75 Lokiarchaeum sp. GC14_75] and [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YEE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5YEE FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=LAB:LATRUNCULIN+B'>LAB</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.81Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Lokiarch_47830 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1538547 Lokiarchaeum sp. GC14_75])</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=LAB:LATRUNCULIN+B'>LAB</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5yee FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yee OCA], [http://pdbe.org/5yee PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yee RCSB], [http://www.ebi.ac.uk/pdbsum/5yee PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yee ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5yee FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yee OCA], [https://pdbe.org/5yee PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5yee RCSB], [https://www.ebi.ac.uk/pdbsum/5yee PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5yee ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/ACTS_RABIT ACTS_RABIT]] Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. | + | [https://www.uniprot.org/uniprot/ACTS_RABIT ACTS_RABIT] Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 5yee" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 5yee" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Actin 3D structures|Actin 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Lokiarchaeum sp. gc14_75]] | + | [[Category: Large Structures]] |
| | + | [[Category: Lokiarchaeum sp. GC14_75]] |
| | [[Category: Oryctolagus cuniculus]] | | [[Category: Oryctolagus cuniculus]] |
| - | [[Category: Akil, C]] | + | [[Category: Akil C]] |
| - | [[Category: Robinson, R C]] | + | [[Category: Robinson RC]] |
| - | [[Category: Actin]]
| + | |
| - | [[Category: Archaea]]
| + | |
| - | [[Category: Lokiarchaea]]
| + | |
| - | [[Category: Structural protein]]
| + | |
| Structural highlights
Function
ACTS_RABIT Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Publication Abstract from PubMed
The origin of the eukaryotic cell is unresolved(1,2). Metagenomics sequencing has recently identified several potential eukaryotic gene homologues in Asgard archaea(3,4), consistent with the hypothesis that the eukaryotic cell evolved from within the Archaea domain. However, many of these eukaryotic-like sequences are highly divergent and the organisms have yet to be imaged or cultivated, which brings into question the extent to which these archaeal proteins represent functional equivalents of their eukaryotic counterparts. Here we show that Asgard archaea encode functional profilins and thereby establish that this archaeal superphylum has a regulated actin cytoskeleton, one of the hallmarks of the eukaryotic cell(5). Loki profilin-1, Loki profilin-2 and Odin profilin adopt the typical profilin fold and are able to interact with rabbit actin-an interaction that involves proteins from species that diverged more than 1.2 billion years ago(6). Biochemical experiments reveal that mammalian actin polymerizes in the presence of Asgard profilins; however, Loki, Odin and Heimdall profilins impede pointed-end elongation. These archaeal profilins also retard the spontaneous nucleation of actin filaments, an effect that is reduced in the presence of phospholipids. Asgard profilins do not interact with polyproline motifs and the profilin-polyproline interaction therefore probably evolved later in the Eukarya lineage. These results suggest that Asgard archaea possess a primordial, polar, profilin-regulated actin system, which may be localized to membranes owing to the sensitivity of Asgard profilins to phospholipids. Because Asgard archaea are also predicted to encode potential eukaryotic-like genes involved in membrane-trafficking and endocytosis(3,4), imaging is now necessary to elucidate whether these organisms are capable of generating eukaryotic-like membrane dynamics that are regulated by actin, such as are observed in eukaryotic cell movement, podosomes and endocytosis.
Genomes of Asgard archaea encode profilins that regulate actin.,Akil C, Robinson RC Nature. 2018 Oct 3. pii: 10.1038/s41586-018-0548-6. doi:, 10.1038/s41586-018-0548-6. PMID:30283132[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Akil C, Robinson RC. Genomes of Asgard archaea encode profilins that regulate actin. Nature. 2018 Oct 3. pii: 10.1038/s41586-018-0548-6. doi:, 10.1038/s41586-018-0548-6. PMID:30283132 doi:http://dx.doi.org/10.1038/s41586-018-0548-6
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