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| | ==Miraculin-like protein from Vitis vinifera== | | ==Miraculin-like protein from Vitis vinifera== |
| - | <StructureSection load='5yh4' size='340' side='right' caption='[[5yh4]], [[Resolution|resolution]] 1.30Å' scene=''> | + | <StructureSection load='5yh4' size='340' side='right'caption='[[5yh4]], [[Resolution|resolution]] 1.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5yh4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Grape Grape]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YH4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YH4 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5yh4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vitis_vinifera Vitis vinifera]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YH4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5YH4 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">VITISV_025776 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=29760 Grape])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5yh4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yh4 OCA], [http://pdbe.org/5yh4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yh4 RCSB], [http://www.ebi.ac.uk/pdbsum/5yh4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yh4 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5yh4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yh4 OCA], [https://pdbe.org/5yh4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5yh4 RCSB], [https://www.ebi.ac.uk/pdbsum/5yh4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5yh4 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/D7TY99_VITVI D7TY99_VITVI] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 5yh4" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 5yh4" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Miraculin|Miraculin]] |
| | + | *[[Trypsin inhibitor 3D structures|Trypsin inhibitor 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Grape]] | + | [[Category: Large Structures]] |
| - | [[Category: Furukawa, N]] | + | [[Category: Vitis vinifera]] |
| - | [[Category: Shimizu-Ibuka, A]] | + | [[Category: Furukawa N]] |
| - | [[Category: Beta-trefoil fold]] | + | [[Category: Shimizu-Ibuka A]] |
| - | [[Category: Hydrolase inhibitor]]
| + | |
| - | [[Category: Kunitz superfamily]]
| + | |
| - | [[Category: Miraculin]]
| + | |
| - | [[Category: Protease inhibitor]]
| + | |
| Structural highlights
Function
D7TY99_VITVI
Publication Abstract from PubMed
The so-called miraculin-like proteins (MLPs) are homologous to miraculin, a homodimeric protein with taste-modifying activity that converts sourness into sweetness. The identity between MLPs and miraculin generally ranges from 30% to 55%, and both MLPs and miraculin are categorized into the Kunitz-type soybean trypsin inhibitor (STI) family. MLP from grape (Vitis vinifera; vvMLP) exhibits significant homology to miraculin (61% identity), suggesting that vvMLP possesses miraculin-like properties. The results of size-exclusion chromatography and sensory analysis illustrated that vvMLP exists as a monomer in solution with no detectable taste-modifying activity. Crystal structure determination revealed that vvMLP exists as a beta-trefoil fold, similarly as other MLPs and Kunitz-type protein inhibitors. The conformation of the loops, including the so-called reactive loop in the STI family, was substantially different between vvMLP and STI. Recombinant vvMLP had inhibitory activity against trypsin (Ki=13.7muM), indicating that the protein can act as a moderate trypsin inhibitor.
Structural and functional analysis of miraculin-like protein from Vitis vinifera.,Ohkura SI, Hori M, Saitoh K, Okuzawa T, Okamoto I, Furukawa N, Shimizu-Ibuka A Biochim Biophys Acta Proteins Proteom. 2018 Nov;1866(11):1125-1130. doi:, 10.1016/j.bbapap.2018.08.009. Epub 2018 Aug 27. PMID:30282610[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Ohkura SI, Hori M, Saitoh K, Okuzawa T, Okamoto I, Furukawa N, Shimizu-Ibuka A. Structural and functional analysis of miraculin-like protein from Vitis vinifera. Biochim Biophys Acta Proteins Proteom. 2018 Nov;1866(11):1125-1130. doi:, 10.1016/j.bbapap.2018.08.009. Epub 2018 Aug 27. PMID:30282610 doi:http://dx.doi.org/10.1016/j.bbapap.2018.08.009
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