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| | ==Structure of C. crescentus GapR-DNA== | | ==Structure of C. crescentus GapR-DNA== |
| - | <StructureSection load='6cg8' size='340' side='right' caption='[[6cg8]], [[Resolution|resolution]] 2.30Å' scene=''> | + | <StructureSection load='6cg8' size='340' side='right'caption='[[6cg8]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6cg8]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Cauvi Cauvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CG8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CG8 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6cg8]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Caulobacter_vibrioides Caulobacter vibrioides] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CG8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6CG8 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">B7Z12_12435, CA606_13135 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=155892 CAUVI])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.299Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cg8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cg8 OCA], [http://pdbe.org/6cg8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cg8 RCSB], [http://www.ebi.ac.uk/pdbsum/6cg8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cg8 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6cg8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cg8 OCA], [https://pdbe.org/6cg8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6cg8 RCSB], [https://www.ebi.ac.uk/pdbsum/6cg8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6cg8 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/Y3428_CAUVN Y3428_CAUVN] |
| - | When DNA is unwound during replication, it becomes overtwisted and forms positive supercoils in front of the translocating DNA polymerase. Unless removed or dissipated, this superhelical tension can impede replication elongation. Topoisomerases, including gyrase and topoisomerase IV in bacteria, are required to relax positive supercoils ahead of DNA polymerase but may not be sufficient for replication. Here, we find that GapR, a chromosome structuring protein in Caulobacter crescentus, is required to complete DNA replication. GapR associates in vivo with positively supercoiled chromosomal DNA, and our biochemical and structural studies demonstrate that GapR forms a dimer-of-dimers that fully encircles overtwisted DNA. Further, we show that GapR stimulates gyrase and topo IV to relax positive supercoils, thereby enabling DNA replication. Analogous chromosome structuring proteins that locate to the overtwisted DNA in front of replication forks may be present in other organisms, similarly helping to recruit and stimulate topoisomerases during DNA replication.
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| - | A Bacterial Chromosome Structuring Protein Binds Overtwisted DNA to Stimulate Type II Topoisomerases and Enable DNA Replication.,Guo MS, Haakonsen DL, Zeng W, Schumacher MA, Laub MT Cell. 2018 Sep 7. pii: S0092-8674(18)31045-6. doi: 10.1016/j.cell.2018.08.029. PMID:30220456<ref>PMID:30220456</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
| + | |
| - | <div class="pdbe-citations 6cg8" style="background-color:#fffaf0;"></div>
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| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Cauvi]] | + | [[Category: Caulobacter vibrioides]] |
| - | [[Category: Schumacher, M A]] | + | [[Category: Large Structures]] |
| - | [[Category: Dna binding]] | + | [[Category: Synthetic construct]] |
| - | [[Category: Dna binding protein]] | + | [[Category: Schumacher MA]] |
| - | [[Category: Dna binding protein-dna complex]]
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| - | [[Category: Gapr]]
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