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2rdd
From Proteopedia
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[[Image:2rdd.jpg|left|200px]] | [[Image:2rdd.jpg|left|200px]] | ||
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| - | + | {{STRUCTURE_2rdd| PDB=2rdd | SCENE= }} | |
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'''X-ray crystal structure of AcrB in complex with a novel transmembrane helix.''' | '''X-ray crystal structure of AcrB in complex with a novel transmembrane helix.''' | ||
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| + | ==Overview== | ||
| + | Bacterial drug resistance is a serious concern for human health. Multidrug efflux pumps export a broad variety of substrates out of the cell and thereby convey resistance to the host. In Escherichia coli, the AcrB:AcrA:TolC efflux complex forms a principal transporter for which structures of the individual component proteins have been determined in isolation. Here, we present the X-ray structure of AcrB in complex with a single transmembrane protein, assigned by mass spectrometry as YajC. A specific rotation of the periplasmic porter domain of AcrB is also revealed, consistent with the hypothesized "twist-to-open" mechanism for TolC activation. Growth experiments with yajc-deleted E. coli reveal a modest increase in the organism's susceptibility to beta-lactam antibiotics, but this effect could not conclusively be attributed to the loss of interactions between YajC and AcrB. | ||
==About this Structure== | ==About this Structure== | ||
2RDD is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RDD OCA]. | 2RDD is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RDD OCA]. | ||
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| + | ==Reference== | ||
| + | Crystal structure of AcrB in complex with a single transmembrane subunit reveals another twist., Tornroth-Horsefield S, Gourdon P, Horsefield R, Brive L, Yamamoto N, Mori H, Snijder A, Neutze R, Structure. 2007 Dec;15(12):1663-73. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18073115 18073115] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: Neutze, R.]] | [[Category: Neutze, R.]] | ||
[[Category: Tornroth-Horsefield, S.]] | [[Category: Tornroth-Horsefield, S.]] | ||
| - | [[Category: | + | [[Category: Acrb]] |
| - | [[Category: | + | [[Category: Antiporter]] |
| - | [[Category: | + | [[Category: Drug resistance]] |
| - | [[Category: | + | [[Category: Inner membrane]] |
| - | [[Category: | + | [[Category: Membrane protein]] |
| - | [[Category: | + | [[Category: Membrane protein/transport protein complex]] |
| - | [[Category: | + | [[Category: Multidrug efflux]] |
| - | [[Category: | + | [[Category: Novel transmembrane helix]] |
| - | [[Category: | + | [[Category: Transporter]] |
| - | [[Category: | + | [[Category: Yajc]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 16 23:07:57 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 20:07, 16 April 2008
X-ray crystal structure of AcrB in complex with a novel transmembrane helix.
Overview
Bacterial drug resistance is a serious concern for human health. Multidrug efflux pumps export a broad variety of substrates out of the cell and thereby convey resistance to the host. In Escherichia coli, the AcrB:AcrA:TolC efflux complex forms a principal transporter for which structures of the individual component proteins have been determined in isolation. Here, we present the X-ray structure of AcrB in complex with a single transmembrane protein, assigned by mass spectrometry as YajC. A specific rotation of the periplasmic porter domain of AcrB is also revealed, consistent with the hypothesized "twist-to-open" mechanism for TolC activation. Growth experiments with yajc-deleted E. coli reveal a modest increase in the organism's susceptibility to beta-lactam antibiotics, but this effect could not conclusively be attributed to the loss of interactions between YajC and AcrB.
About this Structure
2RDD is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Crystal structure of AcrB in complex with a single transmembrane subunit reveals another twist., Tornroth-Horsefield S, Gourdon P, Horsefield R, Brive L, Yamamoto N, Mori H, Snijder A, Neutze R, Structure. 2007 Dec;15(12):1663-73. PMID:18073115 Page seeded by OCA on Wed Apr 16 23:07:57 2008
Categories: Escherichia coli | Protein complex | Gourdon, P. | Horsefield, R. | Neutze, R. | Tornroth-Horsefield, S. | Acrb | Antiporter | Drug resistance | Inner membrane | Membrane protein | Membrane protein/transport protein complex | Multidrug efflux | Novel transmembrane helix | Transporter | Yajc
