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2riq

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[[Image:2riq.jpg|left|200px]]
[[Image:2riq.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2riq |SIZE=350|CAPTION= <scene name='initialview01'>2riq</scene>, resolution 1.70&Aring;
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The line below this paragraph, containing "STRUCTURE_2riq", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:Zn+Binding+Site+For+Residue+A+1'>AC1</scene>, <scene name='pdbsite=AC2:Eoh+Binding+Site+For+Residue+A+2'>AC2</scene>, <scene name='pdbsite=AC3:Gol+Binding+Site+For+Residue+A+3'>AC3</scene>, <scene name='pdbsite=AC4:Gol+Binding+Site+For+Residue+A+4'>AC4</scene> and <scene name='pdbsite=AC5:Gol+Binding+Site+For+Residue+A+5'>AC5</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=EOH:ETHANOL'>EOH</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/NAD(+)_ADP-ribosyltransferase NAD(+) ADP-ribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.30 2.4.2.30] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= PARP1, ADPRT, PPOL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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-->
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|DOMAIN=
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{{STRUCTURE_2riq| PDB=2riq | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2riq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2riq OCA], [http://www.ebi.ac.uk/pdbsum/2riq PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2riq RCSB]</span>
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}}
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'''Crystal Structure of the Third Zinc-binding domain of human PARP-1'''
'''Crystal Structure of the Third Zinc-binding domain of human PARP-1'''
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A Third Zinc-binding Domain of Human Poly(ADP-ribose) Polymerase-1 Coordinates DNA-dependent Enzyme Activation., Langelier MF, Servent KM, Rogers EE, Pascal JM, J Biol Chem. 2008 Feb 15;283(7):4105-14. Epub 2007 Nov 30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18055453 18055453]
A Third Zinc-binding Domain of Human Poly(ADP-ribose) Polymerase-1 Coordinates DNA-dependent Enzyme Activation., Langelier MF, Servent KM, Rogers EE, Pascal JM, J Biol Chem. 2008 Feb 15;283(7):4105-14. Epub 2007 Nov 30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18055453 18055453]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: NAD(+) ADP-ribosyltransferase]]
 
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Langelier, M F.]]
[[Category: Langelier, M F.]]
[[Category: Pascal, J M.]]
[[Category: Pascal, J M.]]
[[Category: Servent, K M.]]
[[Category: Servent, K M.]]
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[[Category: adp-ribosylation]]
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[[Category: Adp-ribosylation]]
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[[Category: dna damage]]
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[[Category: Dna damage]]
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[[Category: dna repair]]
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[[Category: Dna repair]]
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[[Category: dna-binding]]
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[[Category: Dna-binding]]
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[[Category: glycosyltransferase]]
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[[Category: Glycosyltransferase]]
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[[Category: metal-binding]]
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[[Category: Metal-binding]]
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[[Category: nad]]
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[[Category: Nad]]
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[[Category: nucleus]]
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[[Category: Nucleus]]
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[[Category: phosphorylation]]
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[[Category: Phosphorylation]]
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[[Category: polymorphism]]
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[[Category: Polymorphism]]
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[[Category: transferase]]
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[[Category: Transferase]]
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[[Category: zinc]]
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[[Category: Zinc]]
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[[Category: zinc-finger]]
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[[Category: Zinc-finger]]
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[[Category: zn finger]]
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[[Category: Zn finger]]
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[[Category: zn ribbon]]
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[[Category: Zn ribbon]]
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[[Category: zn-binding domain]]
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[[Category: Zn-binding domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 16:59:12 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:00:56 2008''
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Revision as of 13:59, 4 May 2008

Image:2riq.jpg

Template:STRUCTURE 2riq

Crystal Structure of the Third Zinc-binding domain of human PARP-1


Overview

Poly(ADP-ribose) polymerase-1 (PARP-1) is a chromatin-associated enzyme with multiple cellular functions, including DNA repair, transcriptional regulation, and cell signaling. PARP-1 has a modular architecture with six independent domains comprising the 113-kDa polypeptide. Two zinc finger domains at the N terminus of PARP-1 bind to DNA and thereby activate the catalytic domain situated at the C terminus of the enzyme. The tight coupling of DNA binding and catalytic activities is critical to the cellular regulation of PARP-1 function; however, the mechanism for coordinating these activities remains an unsolved problem. Here, we demonstrate using spectroscopic and crystallographic analysis that human PARP-1 has a third zinc-binding domain. Biochemical mutagenesis and deletion analysis indicate that this region mediates interdomain contacts important for DNA-dependent enzyme activation. The crystal structure of the third zinc-binding domain reveals a zinc ribbon fold and suggests conserved residues that could form interdomain contacts. The new zinc-binding domain self-associates in the crystal lattice to form a homodimer with a head-totail arrangement. The structure of the homodimer provides a scaffold for assembling the activated state of PARP-1 and suggests a mechanism for coupling the DNA binding and catalytic functions of PARP-1.

About this Structure

2RIQ is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

A Third Zinc-binding Domain of Human Poly(ADP-ribose) Polymerase-1 Coordinates DNA-dependent Enzyme Activation., Langelier MF, Servent KM, Rogers EE, Pascal JM, J Biol Chem. 2008 Feb 15;283(7):4105-14. Epub 2007 Nov 30. PMID:18055453 Page seeded by OCA on Sun May 4 16:59:12 2008

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