This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2rmi
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:2rmi.gif|left|200px]] | [[Image:2rmi.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_2rmi", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | | | + | --> |
| - | + | {{STRUCTURE_2rmi| PDB=2rmi | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''3D NMR structure of astressin''' | '''3D NMR structure of astressin''' | ||
| Line 19: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2RMI is a [[Single protein]] structure | + | 2RMI is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RMI OCA]. |
==Reference== | ==Reference== | ||
| Line 29: | Line 26: | ||
[[Category: Rivier, J.]] | [[Category: Rivier, J.]] | ||
[[Category: Royappa, G C.R.]] | [[Category: Royappa, G C.R.]] | ||
| - | [[Category: | + | [[Category: Astressin]] |
| - | [[Category: | + | [[Category: Crf antagonist]] |
| - | [[Category: | + | [[Category: Neuropeptide]] |
| - | [[Category: | + | [[Category: Nmr]] |
| - | [[Category: | + | [[Category: Urocortin]] |
| - | [[Category: | + | [[Category: Urotensin]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 17:10:34 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 14:10, 4 May 2008
3D NMR structure of astressin
Overview
The C-terminally amidated CRF antagonist astressin binds to CRF-R1 or CRF-R2 receptors with low nanomolar affinity while the corresponding astressin-acid has >100 times less affinity. To understand the role of the amide group in binding, the conformations of astressin-amide and astressin-acid were studied in DMSO using NMR techniques. The 3D NMR structures show that the backbones of both analogs prefer an alpha-helical conformation, with a small kink around Gln(26). However, astressin-amide has a well-defined helical structure from Leu(27) to Ile(41) and a conformation very similar to the bioactive conformation reported by our group (Grace et al., Proc Natl Acad Sci USA 2007, 104, 4858-4863). In contrast, astressin-acid has an irregular helical conformation from Arg(35) onward, including a rearrangement of the side chains in that region. This structural difference highlights the crucial role of the C-terminal amidation for stabilization of astressin's bioactive conformation.
About this Structure
2RMI is a Single protein structure. Full crystallographic information is available from OCA.
Reference
Astressin-amide and astressin-acid are structurally different in dimethylsulfoxide., Grace CR, Cervini L, Gulyas J, Rivier J, Riek R, Biopolymers. 2007 Oct 5-15;87(2-3):196-205. PMID:17657708 Page seeded by OCA on Sun May 4 17:10:34 2008
Categories: Single protein | Cervini, L. | Gulyas, J. | Riek, R. | Rivier, J. | Royappa, G C.R. | Astressin | Crf antagonist | Neuropeptide | Nmr | Urocortin | Urotensin
