2sfp

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[[Image:2sfp.gif|left|200px]]
[[Image:2sfp.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2sfp |SIZE=350|CAPTION= <scene name='initialview01'>2sfp</scene>, resolution 1.900&Aring;
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The line below this paragraph, containing "STRUCTURE_2sfp", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5&#39;-PHOSPHATE'>PLP</scene>, <scene name='pdbligand=PPI:PROPANOIC+ACID'>PPI</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Alanine_racemase Alanine racemase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.1.1 5.1.1.1] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_2sfp| PDB=2sfp | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2sfp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2sfp OCA], [http://www.ebi.ac.uk/pdbsum/2sfp PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2sfp RCSB]</span>
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}}
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'''ALANINE RACEMASE WITH BOUND PROPIONATE INHIBITOR'''
'''ALANINE RACEMASE WITH BOUND PROPIONATE INHIBITOR'''
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[[Category: Petsko, G A.]]
[[Category: Petsko, G A.]]
[[Category: Ringe, D.]]
[[Category: Ringe, D.]]
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[[Category: alanine]]
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[[Category: Alanine]]
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[[Category: isomerase]]
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[[Category: Isomerase]]
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[[Category: pyridoxal phosphate]]
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[[Category: Pyridoxal phosphate]]
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[[Category: racemase]]
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[[Category: Racemase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 17:18:15 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:02:52 2008''
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Revision as of 14:18, 4 May 2008

Image:2sfp.gif

Template:STRUCTURE 2sfp

ALANINE RACEMASE WITH BOUND PROPIONATE INHIBITOR


Overview

The structure of alanine racemase from Bacillus stearothermophilus with the inhibitor propionate bound in the active site was determined by X-ray crystallography to a resolution of 1.9 A. The enzyme is a homodimer in solution and crystallizes with a dimer in the asymmetric unit. Both active sites contain a pyridoxal 5'-phosphate (PLP) molecule in aldimine linkage to Lys39 as a protonated Schiff base, and the pH-independence of UV-visible absorption spectra suggests that the protonated PLP-Lys39 Schiff base is the reactive form of the enzyme. The carboxylate group of propionate bound in the active site makes numerous interactions with active-site residues, defining the substrate binding site of the enzyme. The propionate-bound structure therefore approximates features of the Michaelis complex formed between alanine racemase and its amino acid substrate. The structure also provides evidence for the existence of a carbamate formed on the side-chain amino group of Lys129, stabilized by interactions with one of the residues interacting with the carboxylate group of propionate, Arg136. We propose that this novel interaction influences both substrate binding and catalysis by precisely positioning Arg136 and modulating its charge.

About this Structure

2SFP is a Single protein structure of sequence from Geobacillus stearothermophilus. Full crystallographic information is available from OCA.

Reference

Structure of a Michaelis complex analogue: propionate binds in the substrate carboxylate site of alanine racemase., Morollo AA, Petsko GA, Ringe D, Biochemistry. 1999 Mar 16;38(11):3293-301. PMID:10079072 Page seeded by OCA on Sun May 4 17:18:15 2008

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