2sob
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:2sob.gif|left|200px]] | [[Image:2sob.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_2sob", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_2sob| PDB=2sob | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''SN-OB, OB-FOLD SUB-DOMAIN OF STAPHYLOCOCCAL NUCLEASE, NMR, 10 STRUCTURES''' | '''SN-OB, OB-FOLD SUB-DOMAIN OF STAPHYLOCOCCAL NUCLEASE, NMR, 10 STRUCTURES''' | ||
| Line 19: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2SOB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. This structure supersedes the now removed PDB entry | + | 2SOB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1sob 1sob]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2SOB OCA]. |
==Reference== | ==Reference== | ||
| Line 30: | Line 27: | ||
[[Category: Gittis, A G.]] | [[Category: Gittis, A G.]] | ||
[[Category: Shortle, D.]] | [[Category: Shortle, D.]] | ||
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 17:20:20 2008'' | |
| - | + | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 14:20, 4 May 2008
SN-OB, OB-FOLD SUB-DOMAIN OF STAPHYLOCOCCAL NUCLEASE, NMR, 10 STRUCTURES
Overview
Similar folds often occur in proteins with dissimilar sequences. The OB-fold forms a part of the structures of at least seven non-homologous proteins that share either oligonucleotide or oligosaccharide binding functions. A 1-103 fragment corresponding to the OB-fold of the 149 amino acid residue staphylococcal nuclease gives NMR spectra characteristic of an unfolded protein, i.e. the wild-type nuclease sequence is insufficient to maintain a stable tertiary structure in the absence of the C-terminal one-third of this single-domain protein. By contrast, the 1-103 fragment of nuclease with the mutations Val66Leu and Gly88Val adopts a stable tertiary structure. The NMR solution structure of this latter fragment is a close variation of the OB-fold found in the X-ray structure of the parent protein. The Val66Leu and Gly88Val mutations appear to stabilize tertiary structure by consolidating the hydrophobic core of the nuclease OB-fold sub-domain. Taken together, these results suggest that recurrent structural motifs such as the OB-fold may in some cases represent vestiges of autonomous folding units that, during evolution, have become integrated into more complex cooperative folding domains.
About this Structure
2SOB is a Single protein structure of sequence from Staphylococcus aureus. This structure supersedes the now removed PDB entry 1sob. Full crystallographic information is available from OCA.
Reference
NMR structure of a stable "OB-fold" sub-domain isolated from staphylococcal nuclease., Alexandrescu AT, Gittis AG, Abeygunawardana C, Shortle D, J Mol Biol. 1995 Jul 7;250(2):134-43. PMID:7608966 Page seeded by OCA on Sun May 4 17:20:20 2008
