2uv1
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:2uv1.gif|left|200px]] | [[Image:2uv1.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_2uv1", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | | | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | + | --> | |
| - | + | {{STRUCTURE_2uv1| PDB=2uv1 | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''HEXAGONAL CRYSTAL FORM OF GAMS FROM BACTERIOPHAGE LAMBDA.''' | '''HEXAGONAL CRYSTAL FORM OF GAMS FROM BACTERIOPHAGE LAMBDA.''' | ||
| Line 29: | Line 26: | ||
[[Category: Saikrishnan, K.]] | [[Category: Saikrishnan, K.]] | ||
[[Category: Wigley, D B.]] | [[Category: Wigley, D B.]] | ||
| - | [[Category: | + | [[Category: Bacteriophage lambda]] |
| - | [[Category: | + | [[Category: Inhibitor]] |
| - | [[Category: | + | [[Category: Nuclease inhibitor recbcd inhibitor]] |
| - | [[Category: | + | [[Category: Putative dna mimetic]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 17:32:41 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 14:32, 4 May 2008
HEXAGONAL CRYSTAL FORM OF GAMS FROM BACTERIOPHAGE LAMBDA.
Overview
In Escherichia coli, RecBCD processes double-stranded DNA breaks during the initial stages of homologous recombination. RecBCD contains helicase and nuclease activities, and unwinds and digests the blunt-ended DNA until a specific eight-nucleotide sequence, Chi, is encountered. Chi modulates the nuclease activity of RecBCD and results in a resected DNA end, which is a substrate for RecA during subsequent steps in recombination. RecBCD also acts as a defence mechanism against bacteriophage infection by digesting linear viral DNA present during virus replication or resulting from the action of restriction endonucleases. To avoid this fate, bacteriophage lambda encodes the gene Gam whose product is an inhibitor of RecBCD. Gam has been shown to bind to RecBCD and inhibit its helicase and nuclease activities. We show that Gam inhibits RecBCD by preventing it from binding DNA. We have solved the crystal structure of Gam from two different crystal forms. Using the published crystal structure of RecBCD in complex with DNA we suggest models for the molecular mechanism of Gam-mediated inhibition of RecBCD. We also propose that Gam could be a mimetic of single-stranded, and perhaps also double-stranded, DNA.
About this Structure
2UV1 is a Single protein structure of sequence from Enterobacteria phage lambda. Full crystallographic information is available from OCA.
Reference
The crystal structure of lambda-Gam protein suggests a model for RecBCD inhibition., Court R, Cook N, Saikrishnan K, Wigley D, J Mol Biol. 2007 Aug 3;371(1):25-33. Epub 2007 May 18. PMID:17544443 Page seeded by OCA on Sun May 4 17:32:41 2008
