2uxd

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[[Image:2uxd.gif|left|200px]]
[[Image:2uxd.gif|left|200px]]
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{{Structure
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|PDB= 2uxd |SIZE=350|CAPTION= <scene name='initialview01'>2uxd</scene>, resolution 3.20&Aring;
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|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PAR:PAROMOMYCIN'>PAR</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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{{STRUCTURE_2uxd| PDB=2uxd | SCENE= }}
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|RELATEDENTRY=[[1fjg|1FJG]], [[1gix|1GIX]], [[1i94|1I94]], [[1i95|1I95]], [[1i96|1I96]], [[1i97|1I97]], [[1ibk|1IBK]], [[1ibl|1IBL]], [[1ibm|1IBM]], [[1j5e|1J5E]], [[1jgo|1JGO]], [[1jgp|1JGP]], [[1jgq|1JGQ]], [[1l1u|1L1U]], [[1n32|1N32]], [[1n33|1N33]], [[1n34|1N34]], [[1n36|1N36]], [[1pns|1PNS]], [[1pnx|1PNX]], [[1xmo|1XMO]], [[1xmq|1XMQ]], [[1xnq|1XNQ]], [[1xnr|1XNR]], [[1yl4|1YL4]], [[2b64|2B64]], [[2b9m|2B9M]], [[2b9o|2B9O]], [[2f4v|2F4V]], [[2j00|2J00]], [[2j02|2J02]], [[2uu9|2UU9]], [[2uua|2UUA]], [[2uub|2UUB]], [[2uuc|2UUC]], [[2uxb|2UXB]], [[2uxc|2UXC]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2uxd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uxd OCA], [http://www.ebi.ac.uk/pdbsum/2uxd PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2uxd RCSB]</span>
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}}
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'''CRYSTAL STRUCTURE OF AN EXTENDED TRNA ANTICODON STEM LOOP IN COMPLEX WITH ITS COGNATE MRNA CGGG IN THE CONTEXT OF THE THERMUS THERMOPHILUS 30S SUBUNIT.'''
'''CRYSTAL STRUCTURE OF AN EXTENDED TRNA ANTICODON STEM LOOP IN COMPLEX WITH ITS COGNATE MRNA CGGG IN THE CONTEXT OF THE THERMUS THERMOPHILUS 30S SUBUNIT.'''
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[[Category: Suzuki, T.]]
[[Category: Suzuki, T.]]
[[Category: 30s ribosomal subunit]]
[[Category: 30s ribosomal subunit]]
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[[Category: a site]]
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[[Category: A site]]
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[[Category: anticodon]]
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[[Category: Anticodon]]
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[[Category: coiled coil]]
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[[Category: Decoding]]
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[[Category: frameshift]]
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[[Category: Frameshift]]
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[[Category: frameshift suppressor trna]]
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[[Category: Messenger rna]]
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[[Category: stem-loop]]
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[[Category: Transfer rna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 17:44:13 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:05:48 2008''
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Revision as of 14:44, 4 May 2008

Image:2uxd.gif

Template:STRUCTURE 2uxd

CRYSTAL STRUCTURE OF AN EXTENDED TRNA ANTICODON STEM LOOP IN COMPLEX WITH ITS COGNATE MRNA CGGG IN THE CONTEXT OF THE THERMUS THERMOPHILUS 30S SUBUNIT.


Overview

During translation, some +1 frameshift mRNA sites are decoded by frameshift suppressor tRNAs that contain an extra base in their anticodon loops. Similarly engineered tRNAs have been used to insert nonnatural amino acids into proteins. Here, we report crystal structures of two anticodon stem-loops (ASLs) from tRNAs known to facilitate +1 frameshifting bound to the 30S ribosomal subunit with their cognate mRNAs. ASL(CCCG) and ASL(ACCC) (5'-3' nomenclature) form unpredicted anticodon-codon interactions where the anticodon base 34 at the wobble position contacts either the fourth codon base or the third and fourth codon bases. In addition, we report the structure of ASL(ACGA) bound to the 30S ribosomal subunit with its cognate mRNA. The tRNA containing this ASL was previously shown to be unable to facilitate +1 frameshifting in competition with normal tRNAs (Hohsaka et al. 2001), and interestingly, it displays a normal anticodon-codon interaction. These structures show that the expanded anticodon loop of +1 frameshift promoting tRNAs are flexible enough to adopt conformations that allow three bases of the anticodon to span four bases of the mRNA. Therefore it appears that normal triplet pairing is not an absolute constraint of the decoding center.

About this Structure

2UXD is a Protein complex structure of sequences from Thermus thermophilus. Full crystallographic information is available from OCA.

Reference

Structures of tRNAs with an expanded anticodon loop in the decoding center of the 30S ribosomal subunit., Dunham CM, Selmer M, Phelps SS, Kelley AC, Suzuki T, Joseph S, Ramakrishnan V, RNA. 2007 Jun;13(6):817-23. Epub 2007 Apr 6. PMID:17416634 Page seeded by OCA on Sun May 4 17:44:13 2008

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OCA

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