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2uyd

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[[Image:2uyd.jpg|left|200px]]
[[Image:2uyd.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2uyd |SIZE=350|CAPTION= <scene name='initialview01'>2uyd</scene>, resolution 2.70&Aring;
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The line below this paragraph, containing "STRUCTURE_2uyd", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:Hem+Binding+Site+For+Chain+X'>AC1</scene>, <scene name='pdbsite=AC2:Zn+Binding+Site+For+Chain+X'>AC2</scene>, <scene name='pdbsite=AC3:Zn+Binding+Site+For+Chain+X'>AC3</scene>, <scene name='pdbsite=AC4:Zn+Binding+Site+For+Chain+X'>AC4</scene>, <scene name='pdbsite=AC5:Zn+Binding+Site+For+Chain+X'>AC5</scene>, <scene name='pdbsite=AC7:Zn+Binding+Site+For+Chain+X'>AC7</scene>, <scene name='pdbsite=AC8:Zn+Binding+Site+For+Chain+X'>AC8</scene>, <scene name='pdbsite=AC9:Zn+Binding+Site+For+Chain+X'>AC9</scene>, <scene name='pdbsite=BC1:Act+Binding+Site+For+Chain+X'>BC1</scene> and <scene name='pdbsite=BC2:Act+Binding+Site+For+Chain+X'>BC2</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_2uyd| PDB=2uyd | SCENE= }}
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|RELATEDENTRY=[[1b2v|1B2V]], [[1dk0|1DK0]], [[1dkh|1DKH]], [[1ybj|1YBJ]], [[2cn4|2CN4]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2uyd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uyd OCA], [http://www.ebi.ac.uk/pdbsum/2uyd PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2uyd RCSB]</span>
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}}
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'''CRYSTAL STRUCTURE OF THE SMHASA MUTANT H83A'''
'''CRYSTAL STRUCTURE OF THE SMHASA MUTANT H83A'''
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[[Category: Izadi-Pruneyre, N.]]
[[Category: Izadi-Pruneyre, N.]]
[[Category: Lecroisey, A.]]
[[Category: Lecroisey, A.]]
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[[Category: heme]]
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[[Category: Heme]]
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[[Category: heme acquisition system]]
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[[Category: Heme acquisition system]]
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[[Category: heme binding]]
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[[Category: Heme binding]]
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[[Category: hemophore]]
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[[Category: Hemophore]]
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[[Category: iron]]
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[[Category: Iron]]
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[[Category: iron ligation]]
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[[Category: Iron ligation]]
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[[Category: metal-binding]]
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[[Category: Metal-binding]]
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[[Category: metal-binding protein]]
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[[Category: Metal-binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 17:48:34 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:06:15 2008''
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Revision as of 14:48, 4 May 2008

Image:2uyd.jpg

Template:STRUCTURE 2uyd

CRYSTAL STRUCTURE OF THE SMHASA MUTANT H83A


Overview

Heme carrier HasA has a unique type of histidine/tyrosine heme iron ligation in which the iron ion is in a thermally driven two spin state equilibrium. We recently suggested that the H-bonding between Y75 and the invariantly conserved residue H83 modulates the strength of the Fe-Y75 bond. To unravel the role of H83, we characterize the iron ligation and the electronic properties of both wild type and H83A mutant by a variety of spectroscopic techniques. While H83 in wild type modulates the strength of the Tyr-iron bond, its removal causes detachment of the tyrosine ligand, thus giving rise to a series of pH dependent equilibria among species with different axial ligation. The five coordinated species detected at physiological pH may represent a possible intermediate of the heme transfer mechanism to the receptor.

About this Structure

2UYD is a Single protein structure of sequence from Serratia marcescens. Full crystallographic information is available from OCA.

Reference

Deciphering the structural role of histidine 83 for heme binding in hemophore HasA., Caillet-Saguy C, Turano P, Piccioli M, Lukat-Rodgers GS, Czjzek M, Guigliarelli B, Izadi-Pruneyre N, Rodgers KR, Delepierre M, Lecroisey A, J Biol Chem. 2007 Dec 27;. PMID:18162469 Page seeded by OCA on Sun May 4 17:48:34 2008

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