2v2k
From Proteopedia
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'''THE CRYSTAL STRUCTURE OF FDXA, A 7FE FERREDOXIN FROM MYCOBACTERIUM SMEGMATIS''' | '''THE CRYSTAL STRUCTURE OF FDXA, A 7FE FERREDOXIN FROM MYCOBACTERIUM SMEGMATIS''' | ||
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[[Category: 4fe-4s cluster instability]] | [[Category: 4fe-4s cluster instability]] | ||
[[Category: 7fe ferredoxin]] | [[Category: 7fe ferredoxin]] | ||
| - | [[Category: | + | [[Category: Electron transfer]] |
| - | [[Category: | + | [[Category: Electron transport]] |
| - | [[Category: | + | [[Category: Fe-s cluster]] |
| - | [[Category: | + | [[Category: Iron]] |
| - | [[Category: | + | [[Category: Iron-sulfur]] |
| - | [[Category: | + | [[Category: Metal-binding]] |
| - | [[Category: | + | [[Category: Mycobacterium smegmati]] |
| - | [[Category: | + | [[Category: Mycobacterium tuberculosis]] |
| - | [[Category: | + | [[Category: Transport]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 18:06:41 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 15:06, 4 May 2008
THE CRYSTAL STRUCTURE OF FDXA, A 7FE FERREDOXIN FROM MYCOBACTERIUM SMEGMATIS
Overview
Mycobacterium smegmatis ferredoxin FdxA, which has an orthologue ferredoxin in Mycobacterium tuberculosis, FdxC, contains both one [3Fe-4S] and one [4Fe-4S] cluster. M. smegmatis FdxA has been shown to be a preferred ferredoxin substrate of FprA [F. Fischer, D. Raimondi, A. Aliverti, G. Zanetti, Mycobacterium tuberculosis FprA, a novel bacterial NADPH-ferredoxin reductase, Eur. J. Biochem. 269 (2002) 3005-3013], an adrenodoxin reductase-like flavoprotein of M. tuberculosis, suggesting that M. tuberculosis FdxC could be the physiological partner of the enzyme in providing reducing power to the cytochromes P450. We report here the crystal structure of FdxA at 1.6A resolution (R(factor) 16.5%, R(free) 20.2%). Besides providing an insight on protein architecture for this 106-residue ferredoxin, our crystallographic investigation highlights lability of the [4Fe-4S] center, which is shown to loose a Fe atom during crystal growth. Due to their high similarity (87% sequence identity), the structure here reported can be considered a valuable model for M. tuberculosis FdxC, thus representing a step forward in the study of the complex mycobacterial redox pathways.
About this Structure
2V2K is a Single protein structure of sequence from Mycobacterium smegmatis. Full crystallographic information is available from OCA.
Reference
The crystal structure of FdxA, a 7Fe ferredoxin from Mycobacterium smegmatis., Ricagno S, de Rosa M, Aliverti A, Zanetti G, Bolognesi M, Biochem Biophys Res Commun. 2007 Aug 17;360(1):97-102. Epub 2007 Jun 12. PMID:17577575 Page seeded by OCA on Sun May 4 18:06:41 2008
Categories: Mycobacterium smegmatis | Single protein | Aliverti, A. | Bolognesi, M. | Ricagno, S. | Rosa, M De. | Zanetti, G. | 3fe-4 | 4fe-4 | 4fe-4s cluster instability | 7fe ferredoxin | Electron transfer | Electron transport | Fe-s cluster | Iron | Iron-sulfur | Metal-binding | Mycobacterium smegmati | Mycobacterium tuberculosis | Transport
