2v5k

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[[Image:2v5k.jpg|left|200px]]
[[Image:2v5k.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2v5k |SIZE=350|CAPTION= <scene name='initialview01'>2v5k</scene>, resolution 2.20&Aring;
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The line below this paragraph, containing "STRUCTURE_2v5k", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:Mg+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:Oxm+Binding+Site+For+Chain+A'>AC2</scene>, <scene name='pdbsite=AC3:Mg+Binding+Site+For+Chain+B'>AC3</scene>, <scene name='pdbsite=AC4:Oxm+Binding+Site+For+Chain+B'>AC4</scene>, <scene name='pdbsite=AC5:Po4+Binding+Site+For+Chain+A'>AC5</scene> and <scene name='pdbsite=AC6:Po4+Binding+Site+For+Chain+B'>AC6</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OXM:OXAMIC+ACID'>OXM</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_2v5k| PDB=2v5k | SCENE= }}
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|RELATEDENTRY=[[2v5j|2V5J]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v5k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v5k OCA], [http://www.ebi.ac.uk/pdbsum/2v5k PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2v5k RCSB]</span>
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}}
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'''CLASS II ALDOLASE HPCH- MAGNESIUM- OXAMATE COMPLEX'''
'''CLASS II ALDOLASE HPCH- MAGNESIUM- OXAMATE COMPLEX'''
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[[Category: Rea, D.]]
[[Category: Rea, D.]]
[[Category: Roper, D I.]]
[[Category: Roper, D I.]]
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[[Category: aromatic degradation]]
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[[Category: Aromatic degradation]]
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[[Category: aromatic hydrocarbons catabolism homoprotocatechuate]]
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[[Category: Aromatic hydrocarbons catabolism homoprotocatechuate]]
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[[Category: class ii aldolase]]
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[[Category: Class ii aldolase]]
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[[Category: homoprotocatechuate]]
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[[Category: Homoprotocatechuate]]
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[[Category: lyase]]
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[[Category: Lyase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 18:12:30 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:08:26 2008''
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Revision as of 15:12, 4 May 2008

Image:2v5k.jpg

Template:STRUCTURE 2v5k

CLASS II ALDOLASE HPCH- MAGNESIUM- OXAMATE COMPLEX


Overview

Microorganisms are adept at degrading chemically resistant aromatic compounds. One of the longest and most well characterized aromatic catabolic pathways is the 4-hydroxyphenylacetic acid degradation pathway of Escherichia coli. The final step involves the conversion of 4-hydroxy-2-oxo-heptane-1,7-dioate into pyruvate and succinic semialdehyde. This reaction is catalyzed by 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (HpcH), a member of the divalent metal ion dependent class II aldolase enzymes that have great biosynthetic potential. We have solved the crystal structure of HpcH in the apo form, and with magnesium and the substrate analogue oxamate bound, to 1.6 A and 2.0 A, respectively. Comparison with similar structures of the homologous 2-dehydro-3-deoxygalactarate aldolase, coupled with site-directed mutagenesis data, implicate histidine 45 and arginine 70 as key catalytic residues.

About this Structure

2V5K is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structure and mechanism of HpcH: a metal ion dependent class II aldolase from the homoprotocatechuate degradation pathway of Escherichia coli., Rea D, Fulop V, Bugg TD, Roper DI, J Mol Biol. 2007 Nov 2;373(4):866-76. Epub 2007 Jun 26. PMID:17881002 Page seeded by OCA on Sun May 4 18:12:30 2008

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OCA

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